Abstract：
The aim of the study was to isolate and characterise antimicrobial agents from the leaves of Capparis fascicularis using thin-layer chromatography-direct bioautography (TLC-DB). Capparis fascicularis is a medicinal plant used traditionally to treat various ailments. Previous studies have shown that species of the genus Capparis, contain several classes of secondary metabolites, including sterols. In this study, the leaves of C. fascicularis were extracted with 80% aqueous methanol, and the extract\'s fractions were screened for antimicrobial activity against Staphylococcus aureus ATCC 25923 using a broth micro-dilution assay. The hexane fraction was the most active, with a minimum inhibitory concentration (MIC) of 512 µg/mL. TLC-DB and flash column chromatography of the hexane fraction resulted in the isolation of β-Sitosterol for the first time from C. fascicularis. The compound was characterised using NMR and HRMS.
摘要：
该研究的目的是使用薄层色谱-直接生物自显影（TLC-DB）从Capparis的叶子中分离和表征抗菌剂。Caapprisfascicularis是一种传统上用于治疗各种疾病的药用植物。以前的研究表明，卡帕里斯属的物种，含有几类次级代谢物，包括甾醇。在这项研究中,用80%的甲醇水溶液提取C.fascicularis的叶子，并使用肉汤微量稀释测定法筛选提取物的部分对金黄色葡萄球菌ATCC25923的抗菌活性。己烷馏分是最活跃的，最小抑制浓度（MIC）为512µg/mL。TLC-DB和己烷级分的快速柱色谱首次从C.fascicularis中分离出β-谷甾醇。使用NMR和HRMS表征该化合物。
