PDF(Pubmed)
Abstract：
A cataract is a loss of the transparency of a normal crystalline lens. Multiple factors, including age as the major risk factor for cataracts, can disturb the transparency of the crystalline lens due to cumulative damage from environmental insults to proteins, particularly crystallins. Lens proteins do not turnover, and crystallins undergo extensive post-translational modifications (PTMs) with age in order to interact with each other and maintain their soluble basis for lens transparency. These PTMs include truncation, oxidation, deamidation, acetylation, phosphorylation, and glycosylation. Cataract formation, apart from protein PTMs, involves protein crosslinking, protein insolubilization, and aggregation. Oxidation is a key feature in age-related cataract formation. Due to the role of genetic and environmental factors, as well as its variable clinical presentation, we consider cataracts to be a multifactorial disease. The preliminary results of our study indicate that proteins implicated in the pathway of a structural constituent of the eye lens (BFSP1, BFSP2, CRYAA, CRYAB, CRYBA, CRYBB, CRYGC, CRYGD, CRYGS, KRTs, and VIM), together with AQP1 and AQP5, may also be involved in lens aging.
摘要：
白内障是正常晶状体透明度的损失。多重因素,包括年龄作为白内障的主要危险因素，会干扰晶状体的透明度，由于环境对蛋白质的损害累积，特别是晶状体蛋白。透镜蛋白不周转，和晶状体蛋白随着年龄的增长而经历广泛的翻译后修饰（PTM），以便彼此相互作用并保持其可溶性基础以保持晶状体透明度。这些PTM包括截断，氧化，脱酰胺,乙酰化,磷酸化,和糖基化。白内障形成,除了蛋白质PTM，涉及蛋白质交联，蛋白质不溶解，和聚合。氧化是年龄相关性白内障形成的关键特征。由于遗传和环境因素的作用,以及其可变的临床表现，我们认为白内障是一种多因素疾病。我们研究的初步结果表明，涉及眼晶状体结构成分途径的蛋白质（BFSP1，BFSP2，CRYAA，CRYAB,CRYBA,CRYBB,CRYGC,CRYGD,CRYGS,KRT,和VIM)，与AQP1和AQP5一起，也可能参与晶状体老化。
