PDF(Pubmed)
Abstract：
Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. NaNO3 was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of NaNO3 to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L NaNO3 into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity.
摘要：
果寡糖（FOS）是一种重要的益生元，由转果糖糖基化酶产生。在这项研究中,甘蔗糖蜜被用作生产果转转基酶的底物。NaNO3是酵母提取物的优质氮源，可通过支链淀粉FRR5284生产转果糖糖基化酶，并将NaNO3与酵母提取物氮的比例从1：0降低至1：1导致总转果糖糖基化活性从109.8U/mL降低至82.5U/mL。仅向含100g/L单糖和二糖的糖蜜培养基中添加4.4g/LNaNO3导致总果糖转糖基化活性为123.8U/mL。从摇瓶到1L生物反应器的支链淀粉FRR5284转果糖糖基化酶生产工艺的放大将酶活性和生产率提高到171.7U/mL和3.58U/mL/h，比摇瓶高出39%和108%，分别。蔗糖(500g/L)被用作细胞外的底物,细胞内，和总支链淀粉FRR5284转果糖糖基化酶，最高收率为61%。细胞内,细胞外，和来自不同生产系统的总支链淀粉FRR5284转果糖糖基化酶导致不同的FOS谱，表明FOS谱可以通过调节细胞内和胞外酶比率来控制，因此益生元活性。
