Abstract：
This chapter provides a methodology for evaluating plant health and leaf characteristics using spectral reflectance. It provides a step-by-step guide to using spectrometers for high-resolution point measurements of leaf spectral reflectance and multispectral imaging for capturing spatial data, emphasizing the importance of consistent measurement conditions. The chapter further explores the intricacies of multispectral imaging, including calibration, data collection, and image processing. Finally, this chapter delves into the application of various spectral indices for the quantification of key traits such as pigment content, the status of the xanthophyll cycle, water content, and how to identify spectral regions of interest for further research and development. Serving as a guide for researchers and practitioners in plant science, this chapter provides a straightforward framework for plant health assessment using spectral reflectance.
摘要：
本章提供了一种使用光谱反射率评估植物健康和叶片特征的方法。它提供了使用光谱仪进行叶片光谱反射率的高分辨率点测量和多光谱成像以捕获空间数据的分步指南，强调一致测量条件的重要性。本章进一步探讨了多光谱成像的复杂性，包括校准,数据收集，和图像处理。最后,本章深入研究了各种光谱指数在色素含量等关键性状定量中的应用，叶黄素循环的状态，含水量,以及如何识别感兴趣的光谱区域以进行进一步的研究和开发。作为植物科学研究人员和从业人员的指南，本章为使用光谱反射率的植物健康评估提供了一个简单的框架。
