METHODS: This retrospective cohort study evaluated embryo development (n = 371) using time-lapse imaging; 94 blastocysts underwent preimplantation genetic testing for aneuploidy (PGT-A).
RESULTS: The embryos were classified as fully (Group 1, n = 194) or partially (Group 2, n = 177) compacted. Group 1 had significantly higher proportions of good- and average-quality blastocysts than Group 2 (21.6% vs. 3.4%, p = 0.001; 47.9% vs. 26.6%, p = 0.001, respectively). The time from the morula stage to the beginning and completion of compaction and blastocyst formation was significantly shorter in Group 1 than in Group 2 (78.6 vs. 82.4 h, p = 0.001; 87.0 vs. 92.2 h, p = 0.001; 100.2 vs. 103.7 h, p = 0.017, respectively). Group 1 embryos had larger surface areas than Group 2 embryos at various time points following blastocyst formation. Group 1 blastocysts had significantly higher average expansion rates than Group 2 blastocysts (653.6 vs. 499.2 μm2/h, p = 0.001). PGT-A revealed a higher proportion of euploid embryos in Group 1 than in Group 2 (47.2% vs. 36.6%, p = 0.303).
CONCLUSIONS: Time-lapse microscopy uncovered a positive relationship between compaction and blastocyst quality and its association with embryo ploidy. Hence, compaction evaluation should be prioritized before blastocyst selection for transfer or cryopreservation.
方法:这项回顾性队列研究使用延时成像评估了胚胎发育(n=371);94个胚泡接受了非整倍性植入前遗传学测试(PGT-A)。
结果:胚胎被分类为完全(组1,n=194)或部分(组2,n=177)压缩。第1组的良好和平均质量胚泡比例明显高于第2组(21.6%vs.3.4%,p=0.001;47.9%vs.26.6%,分别为p=0.001)。第1组从桑态度期到开始和完成紧实和胚泡形成的时间明显短于第2组(78.6vs.82.4h,p=0.001;87.0vs.92.2h,p=0.001;100.2vs.103.7h,分别为p=0.017)。在胚泡形成后的不同时间点,组1胚胎具有比组2胚胎更大的表面积。第1组囊胚的平均扩增率明显高于第2组囊胚(653.6vs.499.2μm2/h,p=0.001)。PGT-A显示第1组的整倍体胚胎比例高于第2组(47.2%vs.36.6%,p=0.303)。
结论:延时显微镜揭示了紧实与胚泡质量之间的正相关关系及其与胚胎倍性的关联。因此,在选择移植或冷冻保存的胚泡之前,应优先进行压实评估。