PDF(Pubmed)
Abstract：
Chinchillas are a relatively novel research model compared with other rodent species. They require special considerations when it comes to their husbandry and daily care. Chinchillas tend to be shy animals that are well adapted to masking clinical signs of illness. These characteristics can make them a difficult species to maintain in a research setting. The authors\' institution has maintained chinchillas and established standardized daily animal care procedures for them. Chinchillas are most commonly used for auditory research. They are often used to study the mechanism of different induced auditory conditions or injuries as well as exploration for potential alleviating treatments. Often, tested therapeutics have demonstrated potentially beneficial effects but have not been applied in the specific condition or injury of interest. The development of new applications for therapeutics can lead to groundbreaking discoveries, but testing of new therapeutic applications is often initially performed in an animal model without knowing how the therapeutic will behave in the species. During testing, unexpected adverse events may manifest that require more focused monitoring and supportive care. This scenario occurred when adverse effects were observed in a chinchilla blast-injury model after receiving an acylated glucagon-like peptide-1 (GLP-1) receptor agonist. The study involved evaluation of this therapeutic over an extended amount of time after inducing a controlled pressurized blast-injury followed by multiple repeated hearing tests under anesthesia. Chinchillas enrolled in the study exhibited several clinical signs including weight loss, lethargy, labored breathing, neurologic abnormalities, decreased appetite or decreased fecal output, and otitis. Five primary abnormalities were reported on pathology: aspiration pneumonia, hepatic steatosis, right ventricular dilation, pancreatitis, and tubulointerstitial nephritis. Initially abnormal clinical signs, early mortality rates, and pathology were attributed to multiple anesthetic events. However, a retrospective analysis evaluating the association of different study variable exposures in a stratified comparison demonstrated that the early mortality rates were actually associated with the therapeutic drug given for the first time in this species. In this study, we describe the detailed findings of the retrospective analysis and explore different strategies that can be incorporated to maintain good animal welfare and decrease early animal loss.
摘要：
与其他啮齿动物相比，龙猫是一种相对新颖的研究模型。当涉及到他们的饲养和日常护理时，他们需要特别的考虑。龙猫往往是害羞的动物，非常适合掩盖临床疾病的迹象。这些特征使它们成为研究环境中难以维持的物种。作者机构维护了龙猫，并为它们建立了标准化的日常动物护理程序。龙猫最常用于听觉研究。它们通常用于研究不同诱发的听觉状况或损伤的机制，以及探索潜在的缓解治疗方法。通常,测试的治疗剂已显示出潜在的有益效果，但尚未应用于特定的疾病或目标损伤。治疗学新应用的发展可以带来突破性的发现，但是新的治疗应用的测试通常最初是在动物模型中进行的，而不知道治疗在物种中的表现。在测试过程中,意外不良事件可能需要更集中的监测和支持治疗.当在接受酰化胰高血糖素样肽-1（GLP-1）受体激动剂后，在栗鼠爆炸伤模型中观察到不良反应时，就会发生这种情况。该研究涉及在诱导受控加压爆炸伤后的长时间内评估这种治疗方法，然后在麻醉下进行多次重复听力测试。参与研究的龙猫表现出几种临床症状，包括体重减轻，嗜睡,费力的呼吸，神经系统异常，食欲下降或粪便排出量减少，还有中耳炎.病理报告了五种主要异常：吸入性肺炎，肝脂肪变性,右心室扩张,胰腺炎,和肾小管间质性肾炎。最初的异常临床体征，早期死亡率,和病理学归因于多个麻醉事件。然而,在分层比较中评估不同研究变量暴露相关性的回顾性分析表明,早期死亡率实际上与该物种首次使用的治疗药物相关.在这项研究中,我们描述了回顾性分析的详细结果，并探索了可以纳入的不同策略，以保持良好的动物福利和减少早期动物损失。
