Abstract：
Abortion caused by the parasite Neospora caninum is an important threat to the livestock industry worldwide. Trophoblasts and caruncular cells play major roles in initiating innate immune responses and controlling parasite infection at the fetal-maternal interface. In the present study, bovine uterine epithelial cells (BUECs) and bovine trophoblastic (BT) cells treated with bovine interferon-gamma (IFN-γ), IFN-alpha (IFN-α) and IFN-tau (IFN-τ) followed by infection with N. caninum were examined by measuring the mRNA expression levels of numerous pregnancy-associated proteins and observing parasite growth to elucidate the host-parasite interaction at the uteroplacental region. N. caninum infection increased the expression of prolactin-related protein 1 (PRP1), pregnancy-associated glycoprotein 1 (PAG1), and cytokines (TNF-α, IL-8 and IL-10) in BUECs and of IL-8 in BT cells. Bovine IFN-γ inhibited IL-8 and TNF-α expression in BUECs and IL-8 in BT cells. In contrast, the expression of the interferon-stimulated gene OAS1 was significantly increased by treatment of the infected BT cells with IFN-γ. However, treatment with bovine IFNs did not inhibit N. caninum growth in either cell line. In conclusion, our results suggest that bovine IFN-γ plays a crucial role in control of pathogenesis in uterus and induction of inflammatory response in the placental region following N. caninum infection, rather than growth inhibition of the parasites.
摘要：
由新孢子虫引起的流产是全球畜牧业的重要威胁。滋养层细胞和软骨细胞在启动先天免疫反应和控制胎儿-母体界面的寄生虫感染中起着重要作用。在本研究中,用牛干扰素-γ（IFN-γ）处理的牛子宫上皮细胞（BUEC）和牛滋养细胞（BT），通过测量许多妊娠相关蛋白的mRNA表达水平并观察寄生虫生长以阐明子宫胎盘区域的宿主-寄生虫相互作用来检查IFN-α（IFN-α）和IFN-tau（IFN-τ），然后感染犬。N.caninum感染增加催乳素相关蛋白1（PRP1）的表达，妊娠相关糖蛋白1（PAG1），和细胞因子(TNF-α，BUEC中的IL-8和IL-10）和BT细胞中的IL-8。牛IFN-γ抑制BUECs中IL-8和TNF-α的表达和BT细胞中IL-8的表达。相比之下,通过用IFN-γ处理感染的BT细胞，干扰素刺激的基因OAS1的表达显着增加。然而,用牛IFN处理没有抑制两种细胞系中的犬N.总之,我们的结果表明，牛IFN-γ在控制子宫的发病机制和诱导的炎症反应在胎盘区域后，而不是抑制寄生虫的生长.
