Abstract：
This study was conducted to isolate and identify the bioactive compounds from the ethanolic extract of Syzygium cumini leaf against Vibrio species through a bioassay-guided fractionation. The ethanol extract was exposed to silica gel chromatography followed by reversed phase HPLC to isolate the most effective fraction against V. parahaemolyticus. Using further UHPLC-orbitrap-ion trap mass spectrometry, five compounds were isolated with broad-spectrum potency against a range of Vibrio species viz. V. parahaemolyticus, V. alginolyticus, V. harveyi, V. vulnificus and V. anguillarum. The IC50 values for the compounds ranged from 8 to 48 µg/mL against the most sensitive species V. vulnificus and 58 to >400 µg/mL against V. alginolyticus. The results of the toxicity tests demonstrated that the compounds were not harmful for shrimp. The study\'s findings indicate that S. cumini leaf extract may contain bioactive molecules that are able to be substituted for antibiotics to treat vibriosis in shrimp farming.
摘要：
进行这项研究是为了通过生物测定指导的分级分离，从藤叶的乙醇提取物中分离和鉴定针对弧菌的生物活性化合物。将乙醇提取物暴露于硅胶色谱，然后进行反相HPLC，以分离出针对副溶血弧菌的最有效级分。使用进一步的UHPLC-轨道阱-离子阱质谱，分离出5种化合物，对一系列弧菌具有广谱效力。五、副溶血病，五、溶藻,V.Harveyi,五、创伤和七叶草。化合物的IC50值对最敏感的病毒弧菌为8至48μg/mL，对溶藻弧菌为58至>400μg/mL。毒性试验的结果表明,该化合物对虾无害。该研究的发现表明，umini叶提取物可能含有生物活性分子，能够代替抗生素治疗对虾养殖中的弧菌病。
