PDF(Pubmed)
Abstract：
Chloroethenes are widely used as solvent in the metal industry and the dry cleaning industry, but their spillage into soil and groundwater due to improper handling has negatively impacted human health. Bioremediation using microorganisms is one of the technologies to clean up soil and groundwater contaminated with chloroethenes. In this study, we examined the bioremediation of chloroethene-contaminated soil using wine pomace extract (WPE). WPE is a liquid containing seven major carboxylic acids and other substances extracted from grape pomace produced in winemaking. WPE clearly promoted the anaerobic bioremediation of chloroethenes. In the tetrachloroethene (PCE) degradation test that used fractions derived from WPE, the water-eluted fraction containing L-lactic acid, L-tartaric acid, and others promoted the dechlorination of PCE, whereas the methanol-eluted fraction containing mainly syringic acid did not. In another PCE degradation test that used L-lactic acid, L-tartaric acid, and syringic acid test solutions, L-lactic acid and L-tartaric acid enhanced the dechlorination of PCE, but syringic acid did not. The results suggest that L-lactic acid and L-tartaric acid in WPE function as hydrogen donors in the anaerobic microbial degradation of chloroethene. This technology realizes environmental remediation through the effective use of food by-products.
摘要：
氯乙烯在金属工业和干洗工业中广泛用作溶剂，但是由于处理不当，它们溢出到土壤和地下水中，对人类健康产生了负面影响。使用微生物的生物修复是清理被氯乙烯污染的土壤和地下水的技术之一。在这项研究中,我们研究了使用葡萄酒果渣提取物（WPE）对氯乙烯污染土壤的生物修复。WPE是一种液体，含有七种主要羧酸和其他从酿酒中生产的葡萄果渣中提取的物质。WPE明显促进了氯乙烯的厌氧生物修复。在使用来自WPE的馏分的四氯乙烯(PCE)降解试验中,含有L-乳酸的水洗脱级分，L-酒石酸,其他人促进了PCE的脱氯，而主要含有丁香酸的甲醇洗脱级分则没有。在另一个使用L-乳酸的PCE降解试验中，L-酒石酸,和丁香酸测试溶液，L-乳酸和L-酒石酸增强了PCE的脱氯，但丁香酸没有。结果表明，WPE中的L-乳酸和L-酒石酸在氯乙烯的厌氧微生物降解中起氢供体的作用。该技术通过有效利用食品副产品实现环境修复。
