PDF(Pubmed)
Abstract：
1,3-Dihydroxyacetone (DHA) is a commercially important chemical and widely used in cosmetics, pharmaceuticals, and food industries as it prevents excessive water evaporation, and provides anti-ultraviolet radiation protection and antioxidant activity. Currently, the industrial production of DHA is based on a biotechnological synthetic route using Gluconobacter oxydans. However, achieving higher production requires more improvements in the synthetic process. In this study, we compared DHA synthesis levels in five industrial wild-type Gluconobacter strains, after which the G. oxydans WSH-003 strain was selected. Then, 16 dehydrogenase genes, unrelated to DHA synthesis, were individually knocked out, with one strain significantly enhancing DHA production, reaching 89.49 g L-1 and 42.27% higher than the wild-type strain. By optimizing the culture media, including seed culture and fermentation media, DHA production was further enhanced. Finally, using an established fed-batch fermentation system, DHA production reached 198.81 g L-1 in a 5 L bioreactor, with a glycerol conversion rate of 82.84%.
摘要：
1,3-二羟基丙酮（DHA）是一种重要的商业化学品，广泛用于化妆品中，制药，和食品工业，因为它可以防止水分过度蒸发，并提供抗紫外线保护和抗氧化活性。目前,DHA的工业生产基于使用氧化葡糖杆菌的生物技术合成路线。然而,实现更高的产量需要在合成过程中进行更多的改进。在这项研究中,我们比较了五种工业野生型葡糖杆菌菌株的DHA合成水平，之后,选择氧化银菌WSH-003菌株。然后，16个脱氢酶基因，与DHA合成无关，被单独击倒，一个菌株显着提高DHA的产量，达到89.49gL-1，比野生型菌株高42.27%。通过优化文化媒介,包括种子培养和发酵培养基，DHA产量进一步提高。最后,使用已建立的补料分批发酵系统，在5升生物反应器中，DHA产量达到198.81gL-1，甘油转化率为82.84%。
