PDF(Pubmed)
Abstract：
Ottonia anisum (O. anisum), belonging to the family Piperaceae, is renowned for its medicinal properties. The plant is rich in alkaloids, terpenoids and flavonoids with recorded bioactivities. The stems, roots, and leaves, of the O. anisum have been extensively used in the folk medicine. Therefore, the present study was conducted to examine the pharmacological activities of O. anisum root extract. Methanolic root extract of O. anisum was assessed for local anesthetic, analgesic, anti-inflammatory and HCl-induced acute lung injury activities in animal models. Local anesthetic activity assessed in frog and guinea pigs through foot withdrawal reflex and intradermal wheal method, respectively, revealed the dose-dependent onset time of anesthesia response. In the case of HCl-induced ALI, the mice group orally administered with O. anisum extract were assessed for bronchoalveolar lavage fluid (BLF) contents, oxidative stress, and proinflammatory molecules. The analysis revealed the reduction in inflammatory molecules, neutrophils, and oxidative stress in the extract treated mice group. In addition, the redox homeostasis, reduced GSH and the catalase activity was found to be restored in the treated groups. Intriguingly, the genes associated with the NFkB expression was found to be downregulated in O. anisum extract treated groups. Moreover, the extract unveiled the significant analgesic and anti-inflammatory activities. Overall, the findings emphasize the clinical applicability of O. anisum extract in the treatment of ALI as well as the potential usage in local anesthetic, analgesic, and anti-inflammatory agents during the treatments.
摘要：
Ottoniaanisum（O.anisum)，属于胡椒科，以其药用特性而闻名。这种植物富含生物碱，具有记录的生物活性的萜类和类黄酮。茎，根,和叶子，在民间医学中广泛使用。因此,进行本研究以检查O。茴香根提取物的药理活性。A.Anisum的甲醇根提取物被评估为局部麻醉药，镇痛药,在动物模型中抗炎和盐酸诱导的急性肺损伤活性。通过脚退缩反射和皮内风团方法评估青蛙和豚鼠的局部麻醉活性，分别,揭示了麻醉反应的剂量依赖性起效时间。在HCl诱导的ALI的情况下，口服给药的小鼠组评估支气管肺泡灌洗液（BLF）含量，氧化应激，和促炎分子。分析显示炎症分子的减少，中性粒细胞，提取物处理小鼠组中的氧化应激。此外,氧化还原稳态，在治疗组中发现降低的GSH和过氧化氢酶活性恢复。有趣的是，发现与NFkB表达相关的基因在O.anisum提取物治疗组中下调。此外，该提取物具有显著的镇痛和抗炎活性。总的来说,研究结果强调了O.anisum提取物在ALI治疗中的临床适用性以及在局部麻醉中的潜在用途，镇痛药,和治疗期间的抗炎药。
