PDF(Pubmed)
Abstract：
BACKGROUND: Biochar ozonization was previously shown to dramatically increase its cation exchange capacity, thus improving its nutrient retention capacity. The potential soil application of ozonized biochar warrants the need for a toxicity study that investigates its effects on microorganisms.
RESULTS: In the study presented here, we found that the filtrates collected from ozonized pine 400 biochar and ozonized rogue biochar did not have any inhibitory effects on the soil environmental bacteria Pseudomonas putida, even at high dissolved organic carbon (DOC) concentrations of 300 ppm. However, the growth of Synechococcus elongatus PCC 7942 was inhibited by the ozonized biochar filtrates at DOC concentrations greater than 75 ppm. Further tests showed the presence of some potential inhibitory compounds (terephthalic acid and p-toluic acid) in the filtrate of non-ozonized pine 400 biochar; these compounds were greatly reduced upon wet-ozonization of the biochar material. Nutrient detection tests also showed that dry-ozonization of rogue biochar enhanced the availability of nitrate and phosphate in its filtrate, a property that may be desirable for soil application.
CONCLUSIONS: Ozonized biochar substances can support soil environmental bacterium Pseudomonas putida growth, since ozonization detoxifies the potential inhibitory aromatic molecules.
RESULTS: In the study presented here, we found that the filtrates collected from ozonized pine 400 biochar and ozonized rogue biochar did not have any inhibitory effects on the soil environmental bacteria Pseudomonas putida, even at high dissolved organic carbon (DOC) concentrations of 300 ppm. However, the growth of Synechococcus elongatus PCC 7942 was inhibited by the ozonized biochar filtrates at DOC concentrations greater than 75 ppm. Further tests showed the presence of some potential inhibitory compounds (terephthalic acid and p-toluic acid) in the filtrate of non-ozonized pine 400 biochar; these compounds were greatly reduced upon wet-ozonization of the biochar material. Nutrient detection tests also showed that dry-ozonization of rogue biochar enhanced the availability of nitrate and phosphate in its filtrate, a property that may be desirable for soil application.
CONCLUSIONS: Ozonized biochar substances can support soil environmental bacterium Pseudomonas putida growth, since ozonization detoxifies the potential inhibitory aromatic molecules.
摘要：
背景：生物炭臭氧化先前被证明可以显着提高其阳离子交换能力，从而提高其营养保留能力。臭氧化生物炭的潜在土壤应用保证了需要进行毒性研究以调查其对微生物的影响。
结果：在这里提出的研究中，我们发现，从臭氧化松400生物炭和臭氧化流氓生物炭收集的滤液对土壤环境细菌恶臭假单胞菌没有任何抑制作用，即使在300ppm的高溶解有机碳（DOC）浓度下。然而,在DOC浓度大于75ppm的情况下，臭氧化生物炭滤液抑制了长长复合球菌PCC7942的生长。进一步的测试表明，在非臭氧化松木400生物炭的滤液中存在一些潜在的抑制性化合物（对苯二甲酸和对甲苯甲酸）；这些化合物在生物炭材料的湿法臭氧化后大大减少。营养检测测试还表明，流氓生物炭的干臭氧化增强了滤液中硝酸盐和磷酸盐的可用性，土壤应用所需的性质。
结论：臭氧生物炭物质可以支持土壤环境细菌恶臭假单胞菌的生长，由于臭氧化使潜在的抑制性芳香分子解毒。
结果：在这里提出的研究中，我们发现，从臭氧化松400生物炭和臭氧化流氓生物炭收集的滤液对土壤环境细菌恶臭假单胞菌没有任何抑制作用，即使在300ppm的高溶解有机碳（DOC）浓度下。然而,在DOC浓度大于75ppm的情况下，臭氧化生物炭滤液抑制了长长复合球菌PCC7942的生长。进一步的测试表明，在非臭氧化松木400生物炭的滤液中存在一些潜在的抑制性化合物（对苯二甲酸和对甲苯甲酸）；这些化合物在生物炭材料的湿法臭氧化后大大减少。营养检测测试还表明，流氓生物炭的干臭氧化增强了滤液中硝酸盐和磷酸盐的可用性，土壤应用所需的性质。
结论：臭氧生物炭物质可以支持土壤环境细菌恶臭假单胞菌的生长，由于臭氧化使潜在的抑制性芳香分子解毒。
