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Abstract：
Although electroconvulsive therapy (ECT) is one of the most effective treatments for severe mood and psychotic disorders, the mechanisms underlying its therapeutic effects remain unknown. Electroconvulsive stimulation (ECS), the animal model for ECT, can be used to investigate the potential therapeutic mechanisms of ECT in rodents. ECS produces numerous effects in the brain, such as increasing levels of growth factors, inducing dendritic sprouting, and stimulating neurogenesis. It also induces high-level expression of immediate early genes (IEGs) that have been implicated in the pathogenesis of schizophrenia, such as early growth response 3 (Egr3) and activity-regulated cytoskeleton-associated protein (Arc), a validated downstream target of Egr3 [1-3]. However, the effect of isoflurane anesthesia preceding ECS on IEG response in mice has not been well characterized. This article provides immunofluorescent data of the activity responsive IEG ARC in the dorsal and ventral dentate gyrus of wildtype (WT) mice following ECS with or without anesthesia, as well as following sham ECS. The data in this article relate to a published article that employed serial ECS in mice to investigate the requirement of Egr3 in the neurobiological effects of this model of ECT [4]. The ability to study the effects of serial ECS has been limited in mice due to high rates of mortality during seizure. Administration of isoflurane anesthesia prior to ECS significantly reduces rodent mortality, irrespective of the number of times ECS is applied [5]. Since general anesthesia is administered to patients prior to ECT, use of isoflurane prior to ECS also more closely models the clinical use of ECT [6].
摘要：
尽管电惊厥疗法（ECT）是治疗严重情绪和精神障碍的最有效方法之一，其治疗效果的潜在机制仍然未知.电惊厥刺激(ECS),ECT的动物模型,可用于研究ECT在啮齿动物中的潜在治疗机制。ECS在大脑中产生许多影响，例如增长因素水平的提高，诱导树突发芽，刺激神经发生。它还诱导与精神分裂症发病机理有关的立即早期基因（IEG）的高水平表达，如早期生长反应3（Egr3）和活性调节细胞骨架相关蛋白（Arc），Egr3[1-3]的验证下游目标。然而,在ECS之前的异氟醚麻醉对小鼠IEG反应的影响尚未得到很好的表征.本文提供了在有或没有麻醉的情况下，野生型（WT）小鼠背侧和腹侧齿状回中活动响应性IEGARC的免疫荧光数据，以及跟随假ECS。本文中的数据与一篇发表的文章有关，该文章在小鼠中采用了系列ECS来研究Egr3在这种ECT模型的神经生物学效应中的需求[4]。由于癫痫发作期间的高死亡率，研究连续ECS作用的能力在小鼠中受到限制。ECS前给予异氟烷麻醉显著降低啮齿动物死亡率，与应用ECS的次数无关[5]。由于在ECT之前对患者进行全身麻醉，在ECS之前使用异氟烷也更密切地模拟了ECT的临床应用[6].
