关键词: GDNF childhood trauma cognitive performance first episode of psychosis (FEP) miRNA-29a premorbid adjustment

来  源:   DOI:10.3389/fpsyt.2024.1320650   PDF(Pubmed)

Abstract:
UNASSIGNED: Schizophrenia involves complex interactions between biological and environmental factors, including childhood trauma, cognitive impairments, and premorbid adjustment. Predicting its severity and progression remains challenging. Biomarkers like glial cell line-derived neurotrophic factor (GDNF) and miRNA-29a may bridge biological and environmental aspects. The goal was to explore the connections between miRNAs and neural proteins and cognitive functioning, childhood trauma, and premorbid adjustment in the first episode of psychosis (FEP).
UNASSIGNED: This study included 19 FEP patients who underwent clinical evaluation with: the Childhood Trauma Questionnaire (CTQ), the Premorbid Adjustment Scale (PAS), the Positive and Negative Syndrome Scale (PANSS), and the Montreal Cognitive Assessment Scale (MoCA). Multiplex assays for plasma proteins were conducted with Luminex xMAP technology. Additionally, miRNA levels were quantitatively determined through RNA extraction, cDNA synthesis, and RT-qPCR on a 7500 Fast Real-Time PCR System.
UNASSIGNED: Among miRNAs, only miR-29a-3p exhibited a significant correlation with PAS-C scores (r = -0.513, p = 0.025) and cognitive improvement (r = -0.505, p = 0.033). Among the analyzed proteins, only GDNF showed correlations with MoCA scores at the baseline and after 3 months (r = 0.533, p = 0.0189 and r = 0.598, p = 0.007), cognitive improvement (r = 0.511, p = 0.025), and CTQ subtests. MIF concentrations correlated with the PAS-C subscale (r = -0.5670, p = 0.011).
UNASSIGNED: GDNF and miR-29a-3p are promising as biomarkers for understanding and addressing cognitive deficits in psychosis. This study links miRNA and MIF to premorbid adjustment and reveals GDNF\'s unique role in connection with childhood trauma.
摘要:
精神分裂症涉及生物和环境因素之间的复杂相互作用,包括童年创伤,认知障碍,和病前调整。预测其严重程度和进展仍然具有挑战性。神经胶质细胞系衍生的神经营养因子(GDNF)和miRNA-29a等生物标志物可以桥接生物学和环境方面。目的是探索miRNA和神经蛋白与认知功能之间的联系,童年创伤,和第一次精神病发作(FEP)的病前调整。
这项研究包括19名FEP患者,他们接受了以下临床评估:儿童创伤问卷(CTQ),病前调整量表(PAS),阳性和阴性综合征量表(PANSS),和蒙特利尔认知评估量表(MoCA)。用LuminexxMAP技术进行血浆蛋白的多重测定。此外,通过RNA提取定量测定miRNA水平,cDNA合成,和在7500快速实时PCR系统上的RT-qPCR。
在miRNAs中,只有miR-29a-3p表现出与PAS-C评分(r=-0.513,p=0.025)和认知改善(r=-0.505,p=0.033)显著相关。在分析的蛋白质中,只有GDNF在基线和3个月后与MoCA评分相关(r=0.533,p=0.0189和r=0.598,p=0.007),认知改善(r=0.511,p=0.025),和CTQ子测试。MIF浓度与PAS-C分量表相关(r=-0.5670,p=0.011)。
GDNF和miR-29a-3p有望作为理解和解决精神病认知缺陷的生物标志物。这项研究将miRNA和MIF与病前调节联系起来,并揭示了GDNF在儿童创伤中的独特作用。
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