UNASSIGNED: This study included 19 FEP patients who underwent clinical evaluation with: the Childhood Trauma Questionnaire (CTQ), the Premorbid Adjustment Scale (PAS), the Positive and Negative Syndrome Scale (PANSS), and the Montreal Cognitive Assessment Scale (MoCA). Multiplex assays for plasma proteins were conducted with Luminex xMAP technology. Additionally, miRNA levels were quantitatively determined through RNA extraction, cDNA synthesis, and RT-qPCR on a 7500 Fast Real-Time PCR System.
UNASSIGNED: Among miRNAs, only miR-29a-3p exhibited a significant correlation with PAS-C scores (r = -0.513, p = 0.025) and cognitive improvement (r = -0.505, p = 0.033). Among the analyzed proteins, only GDNF showed correlations with MoCA scores at the baseline and after 3 months (r = 0.533, p = 0.0189 and r = 0.598, p = 0.007), cognitive improvement (r = 0.511, p = 0.025), and CTQ subtests. MIF concentrations correlated with the PAS-C subscale (r = -0.5670, p = 0.011).
UNASSIGNED: GDNF and miR-29a-3p are promising as biomarkers for understanding and addressing cognitive deficits in psychosis. This study links miRNA and MIF to premorbid adjustment and reveals GDNF\'s unique role in connection with childhood trauma.
这项研究包括19名FEP患者,他们接受了以下临床评估:儿童创伤问卷(CTQ),病前调整量表(PAS),阳性和阴性综合征量表(PANSS),和蒙特利尔认知评估量表(MoCA)。用LuminexxMAP技术进行血浆蛋白的多重测定。此外,通过RNA提取定量测定miRNA水平,cDNA合成,和在7500快速实时PCR系统上的RT-qPCR。
■在miRNAs中,只有miR-29a-3p表现出与PAS-C评分(r=-0.513,p=0.025)和认知改善(r=-0.505,p=0.033)显著相关。在分析的蛋白质中,只有GDNF在基线和3个月后与MoCA评分相关(r=0.533,p=0.0189和r=0.598,p=0.007),认知改善(r=0.511,p=0.025),和CTQ子测试。MIF浓度与PAS-C分量表相关(r=-0.5670,p=0.011)。
■GDNF和miR-29a-3p有望作为理解和解决精神病认知缺陷的生物标志物。这项研究将miRNA和MIF与病前调节联系起来,并揭示了GDNF在儿童创伤中的独特作用。