PDF(Pubmed)
Abstract：
The medicinal plant Portulaca oleraceae has a long history of usage in traditional medicine. Plant extracts have several interesting pharmacological effects but have some drawbacks that can be addressed via capsulation with chitosan. This work set out to do just that tally up the antioxidant effects of a polyphenol-rich P. olerace extract and see how capsulation affected them. The reflux extraction and response surface methodology (RSM) were carried out to optimize the phenolic and flavonoid content of P. oleraceae extract. Additionally, high-resolution mass spectrometry was employed to determine the secondary metabolite present in the extract. The microcapsules of extract-loaded chitosan were prepared using the ionic gelation method and characterized in terms of size, encapsulation efficiency (EE), and morphology of microcapsules. Fourier transform infrared (FTIR) was used to observe the successful production of microcapsules with a principal component analysis (PCA) approach. The antioxidant activity of microcapsules was established using the radical scavenging method. According to RSM, the highest amounts of TPC and TFC were obtained at 72.894 % ethanol, 2.031 h, and 57.384 °C. The compounds were employed from the optimized extract of P. oleraceae including phenolics and flavonoids. The microcapsules were secured with a %EE of 43.56 ± 2.31 %. The characteristics of microcapsules were approved for the obtained product\'s successful synthesis according to the PCA. The microcapsules have antioxidant activity in a concentration-dependent manner (p < 0.0001). The findings of this study underscored the benefits of employing chitosan as a nanocarrier for extract, offering a promising approach to enhance plant-derived therapies.
摘要：
药用植物马齿轮轴科在传统医学中有着悠久的使用历史。植物提取物具有几个令人感兴趣的药理作用,但具有一些可通过用壳聚糖包封来解决的缺点。这项工作旨在总结富含多酚的P.olerace提取物的抗氧化作用，并观察胶囊如何影响它们。采用回流提取法和响应面法(RSM)对苦参提取物中的酚类和黄酮含量进行优化。此外,高分辨率质谱法用于确定提取物中存在的次级代谢产物。用离子凝胶法制备了负载提取物的壳聚糖微胶囊，并对其大小进行了表征，封装效率(EE)，和微胶囊的形态。傅里叶变换红外（FTIR）用于通过主成分分析（PCA）方法观察微胶囊的成功生产。采用自由基清除法建立了微胶囊的抗氧化活性。根据RSM,最高含量的TPC和TFC在72.894%乙醇时获得，2.031小时,和57.384°C。所述化合物来自包括酚类和类黄酮的优选的甘蓝科提取物。微胶囊的%EE为43.56±2.31%。根据PCA，微胶囊的特性被批准用于获得的产品的成功合成。微胶囊具有浓度依赖性的抗氧化活性(p<0.0001)。这项研究的结果强调了使用壳聚糖作为提取物纳米载体的好处，提供了一种有希望的方法来增强植物衍生疗法。
