Abstract：
Microalgal polysaccharides have received much attention due to their potential value in preventing and regulating oxidative damage. This study aims to reveal the mechanisms of regulating oxidative stress and the differences in the yield, structure, and effect of polysaccharides extracted from three microalgae: Golenkinia sp. polysaccharides (GPS), Chlorella sorokiniana polysaccharides (CPS), and Spirulina subsalsa polysaccharides (SPS). Using the same extraction method, GPS, CPS, and SPS were all heteropoly- saccharides composed of small molecular fraction: the monosaccharides mainly comprised galactose (Gal). Among the three, SPS had a higher proportion of small molecular fraction, and a higher proportion of Gal; thus it had the highest yield and antioxidant activity. GPS, CPS, and SPS all showed strong antioxidant activity in vitro, and showed strong ability to regulate oxidative stress, among which SPS was slightly higher. From the analysis of gene expression, the Nrf2-ARE signalling pathway was an important pathway for GPS, CPS, and SPS to regulate cellular oxidative stress. This study provides a theoretical foundation for further research on the utilization of microalgae polysaccharides and product development.
摘要：
微藻多糖因其在预防和调节氧化损伤方面的潜在价值而备受关注。本研究旨在揭示氧化应激调控机制及产量差异,结构,和从三种微藻中提取的多糖的作用：Golenkiniasp。多糖（GPS），小球藻多糖（CPS），和螺旋藻亚莎莎多糖（SPS）。使用相同的提取方法，GPS,CPS,和SPS都是由小分子部分组成的杂多糖:单糖主要包括半乳糖(Gal)。在这三个人中,SPS有较高比例的小分子组分，和更高的Gal比例；因此它具有更高的产量和更高的抗氧化活性。GPS,CPS,SPS在体外均表现出较强的抗氧化活性,并显示出较强的调节氧化应激的能力，其中SPS略高。从基因表达的分析，Nrf2-ARE信号通路是GPS的重要途径，CPS,和SPS调节细胞氧化应激。本研究为进一步研究微藻多糖的利用和产品开发提供了理论基础。
