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Abstract：
This in vitro study aimed to evaluate the feasibility of quantitative light-induced fluorescence (QLF) technology for detecting the presence and severity of microleakage of pit and fissure sealants. The areas of interest (AOIs) were 160 pits and fissures of 40 extracted permanent teeth. Fluorescent images were acquired using a QLF device, and the maximum fluorescence loss ΔFmax of each AOI was analyzed. After staining and cross-sectioning of the teeth, histological dye penetration was scored on a scale of 0 to 3. The relationship between ΔFmax and microleakage depth was analyzed, and the areas under the curve (AUCs) were calculated. The │ΔFmax│ increased as microleakage depth increased. The ΔFmax values of microleakage areas showed a strong significant correlation with the histological scores of dye penetration (r = - 0.72, P = 0.001). AUC analysis showed a high diagnostic accuracy for microleakage depth (AUC = 0.83-0.91). The highest AUC of 0.91 was found when differentiating the outer half microleakage of the sealant (histological score 0 vs. 1-3). QLF technology is effective in assessing the presence and severity of microleakage, suggesting its potential for noninvasive detection and monitoring of sealant microleakage in clinical settings.
摘要：
这项体外研究旨在评估定量光诱导荧光（QLF）技术用于检测凹坑和裂缝密封剂微泄漏的存在和严重程度的可行性。感兴趣的区域（AOI）是40颗拔除的恒牙的160个凹坑和裂缝。使用QLF设备采集荧光图像，并且分析每个AOI的最大荧光损失ΔFmax。染色和横切牙齿后，组织学染料渗透评分为0至3。分析了ΔFmax与微泄漏深度的关系,并计算曲线下面积(AUC)。当微泄漏深度增加时，△Fmax增加。微渗漏区域的ΔFmax值与染料渗透的组织学评分具有很强的显着相关性（r=-0.72，P=0.001）。AUC分析显示出微泄漏深度的高诊断准确性（AUC=0.83-0.91）。区分密封剂的外半微渗漏时，发现最高AUC为0.91（组织学评分0与1-3).QLF技术可有效评估微泄漏的存在和严重程度，提示其在临床环境下非侵入性检测和监测密封剂微渗漏的潜力。
