RESULTS: Thirty buffalo milk samples based on clinical signs and CMT were divided into normal, subclinical, and clinical mastitis. SCC evaluation showed significant differences between the groups. The data analysis demonstrated that the elevation of miR-146a and miR-383 differed substantially between normal, subclinical, and clinical mastitis milk of buffaloes with 100% sensitivity and specificity. The relationship of SCC with miR-146a and miR-383 in normal/healthy and subclinical mastitis was positively correlated.
CONCLUSIONS: The overexpression of miR-146a and miR-383 is associated with inflammation. It can be a valuable prognostic and most sensitive biomarker for early mastitis detection in buffaloes with SCC below 2 lakhs and CMT-ve, enhancing the accuracy of subclinical mastitis diagnosis.
结果:根据临床体征和CMT将30例水牛乳样本分为正常,亚临床,和临床乳腺炎。SCC评估显示两组之间存在显着差异。数据分析表明,miR-146a和miR-383的升高在正常,亚临床,水牛临床乳腺炎乳汁具有100%的敏感性和特异性。正常/健康和亚临床乳腺炎中SCC与miR-146a和miR-383的关系呈正相关。
结论:miR-146a和miR-383的过表达与炎症相关。对于SCC低于2lakhs和CMT-ve的水牛早期乳腺炎检测,它可以是有价值的预后和最敏感的生物标志物。提高亚临床乳腺炎诊断的准确性。