Abstract：
Chemical production wastewater contains large amounts of organic solvents (OSs), which pose a significant threat to the environment. In this study, a 10 g·L-1 styrene oxide tolerant strain with broad-spectrum OSs tolerance was obtained via adaptive laboratory evolution. The mechanisms underlying the high OS tolerance of tolerant strain were investigated by integrating physiological, multi-omics, and genetic engineering analyses. Physiological changes are one of the main factors responsible for the high OS tolerance in mutant strains. Moreover, the P-type ATPase GOX_RS04415 and the LysR family transcriptional regulator GOX_RS04700 were also verified as critical genes for styrene oxide tolerance. The tolerance mechanisms of OSs can be used in biocatalytic chassis cell factories to synthesize compounds and degrade environmental pollutants. This study provides new insights into the mechanisms underlying the toxicological response to OS stress and offers potential targets for enhancing the solvent tolerance of G. oxydans.
摘要：
化工生产废水中含有大量的有机溶剂（OS），对环境构成重大威胁。在这项研究中,通过适应性实验室进化获得了具有广谱OSs耐受性的10g·L-1苯乙烯氧化物耐受菌株。通过整合生理、多组学，和基因工程分析。生理变化是导致突变株OS耐受性高的主要因素之一。此外，P型ATP酶GOX_RS04415和LysR家族转录调节因子GOX_RS04700也被验证为氧化苯乙烯耐受性的关键基因。OSs的耐受机制可用于生物催化底盘细胞工厂合成化合物和降解环境污染物。这项研究为OS应激的毒理学反应的潜在机制提供了新的见解，并为增强氧化甘醇的溶剂耐受性提供了潜在的目标。
