Abstract：
The Pacific oyster Crassostrea gigas is rich in taurine, which is crucial for its adaptation to the fluctuating intertidal environment and presents significant potential in improving taurine nutrition and boosting immunity in humans. Cysteine dioxygenase (CDO) is a key enzyme involved in the initial step of taurine biosynthesis and plays a crucial role in regulating taurine content in the body. In the present study, polymorphisms of CDO gene in C. gigas (CgCDO) and their association with taurine content were evaluated in 198 individuals. A total of 24 single nucleotide polymorphism (SNP) loci were identified in the exonic region of CgCDO gene by direct sequencing. Among these SNPs, c.279G>A and c.287C>A were found to be significantly associated with taurine content, with the GG and AA genotype at the two loci exhibiting enhanced taurine accumulation (p < 0.05). Haplotype analysis revealed that the 279GG/287AA haplotype had the highest taurine content of 29.24 mg/g, while the 279AA/287CC haplotype showed the lowest taurine content of 21.19 mg/g. These results indicated that the SNPs of CgCDO gene could influence the taurine content in C. gigas and have potential applications in the selective breeding of high-taurine varieties.
摘要：
太平洋牡蛎Crassostreagigas富含牛磺酸，这对于适应潮间带波动的环境至关重要，并且在改善牛磺酸营养和增强人类免疫力方面具有巨大潜力。半胱氨酸双加氧酶(CDO)是参与牛磺酸生物合成初始步骤的关键酶,在调节体内牛磺酸含量方面起着至关重要的作用。在本研究中,在198例个体中评估了C.gigas（CgCDO）中CDO基因的多态性及其与牛磺酸含量的关联。通过直接测序在CgCDO基因的外显子区域中总共鉴定了24个单核苷酸多态性（SNP）基因座。在这些SNP中，c.279G>A和c.287C>A被发现与牛磺酸含量显著相关，GG和AA基因型在两个基因座处表现出增强的牛磺酸积累(p<0.05)。单倍型分析显示,279GG/287AA单倍型牛磺酸含量最高,为29.24mg/g,279AA/287CC单倍型牛磺酸含量最低，为21.19mg/g。这些结果表明，CgCDO基因的SNP可以影响C.gigas中牛磺酸的含量，并在高牛磺酸品种的选择育种中具有潜在的应用。
