PDF(Pubmed)
Abstract：
BACKGROUND: Various cell culture platforms that could display native environmental cue-mimicking stimuli were developed, and effects of environmental cues on cell behaviors were studied with the cell culture platforms. Likewise, various cell culture platforms mimicking native trabecular meshwork (TM) composed of juxtacanalicular, corneoscleral and uveal meshwork located in internal scleral sulcus were used to study effects of environmental cues and/or drug treatments on TM cells and glaucoma development. Glaucoma is a disease that could cause blindness, and cause of glaucoma is not clearly identified yet. It appears that aqueous humor (AH) outflow resistance increased by damages on pathway of AH outflow can elevate intraocular pressure (IOP). These overall possibly contribute to development of glaucoma.
METHODS: For the study of glaucoma, static and dynamic cell culture platforms were developed. Particularly, the dynamic platforms exploiting AH outflow-mimicking perfusion or increased IOP-mimicking increased pressure were used to study how perfusion or increased pressure could affect TM cells. Overall, potential mechanisms of glaucoma development, TM structures and compositions, TM cell culture platform types and researches on TM cells and glaucoma development with the platforms were described in this review.
CONCLUSIONS: This will be useful to improve researches on TM cells and develop enhanced therapies targeting glaucoma.
METHODS: For the study of glaucoma, static and dynamic cell culture platforms were developed. Particularly, the dynamic platforms exploiting AH outflow-mimicking perfusion or increased IOP-mimicking increased pressure were used to study how perfusion or increased pressure could affect TM cells. Overall, potential mechanisms of glaucoma development, TM structures and compositions, TM cell culture platform types and researches on TM cells and glaucoma development with the platforms were described in this review.
CONCLUSIONS: This will be useful to improve researches on TM cells and develop enhanced therapies targeting glaucoma.
摘要：
背景：开发了各种可以显示天然环境线索模拟刺激的细胞培养平台，在细胞培养平台上研究了环境线索对细胞行为的影响。同样，各种细胞培养平台模仿由近耳组成的天然小梁网（TM），使用位于巩膜内沟的角巩膜和葡萄膜网来研究环境线索和/或药物治疗对TM细胞和青光眼发展的影响。青光眼是一种可能导致失明的疾病，青光眼的病因尚未明确。看来，房水（AH）流出阻力因AH流出途径的损害而增加，可以升高眼内压（IOP）。这些总体上可能有助于青光眼的发展。
方法：对于青光眼的研究，开发了静态和动态细胞培养平台。特别是,利用模拟AH流出灌注或模拟IOP升高压力的动态平台用于研究灌注或升高压力如何影响TM细胞.总的来说,青光眼发展的潜在机制，TM结构和组成，本文就TM细胞培养平台的类型以及TM细胞与青光眼发展的研究进展作一综述。
结论：这将有助于改善对TM细胞的研究并开发针对青光眼的增强疗法。
方法：对于青光眼的研究，开发了静态和动态细胞培养平台。特别是,利用模拟AH流出灌注或模拟IOP升高压力的动态平台用于研究灌注或升高压力如何影响TM细胞.总的来说,青光眼发展的潜在机制，TM结构和组成，本文就TM细胞培养平台的类型以及TM细胞与青光眼发展的研究进展作一综述。
结论：这将有助于改善对TM细胞的研究并开发针对青光眼的增强疗法。
