RESULTS: We used bioinformatic approaches to identify members of the J. sigillata ARF gene family and analyze their evolutionary relationships, collinearity, cis-acting elements, and tissue-specific expression patterns. The expression patterns of ARF gene family members under natural drought conditions were also analyzed. The J. sigillata ARF gene family contained 31 members, which were unevenly distributed across 16 chromosomes. We constructed a phylogenetic tree of JsARF genes and other plant ARF genes. Cis-acting elements in the promoters of JsARF were predicted. JsARF28 showed higher expressions in both the roots and leaves. A heat map of the transcriptome data of the cluster analysis under drought stress indicated that JsARF3/9/11/17/20/26 are responsive to drought. The expression of the 11 ARF genes varied under PEG treatment and JsARF18 and JsARF20 were significantly up-regulated.
CONCLUSIONS: The interactions between abiotic stresses and plant hormones are supported by our cumulative data, which also offers a theoretical groundwork for comprehending the ARF mechanism and drought resistance in J. sigillata.
结果:我们使用生物信息学方法来鉴定J.sigillataARF基因家族的成员,并分析它们的进化关系,共线性,顺式作用元素,和组织特异性表达模式。同时分析了ARF基因家族成员在自然干旱条件下的表达模式。J.sigillataARF基因家族包含31个成员,在16条染色体上分布不均.我们构建了JsARF基因和其他植物ARF基因的系统发育树。预测了JsARF启动子中的顺式作用元件。JsARF28在根和叶中均显示出较高的表达。干旱胁迫下聚类分析的转录组数据的热图表明JsARF3/9/11/17/20/26对干旱有响应。11种ARF基因的表达在PEG处理下变化,JsARF18和JsARF20显著上调。
结论:非生物胁迫与植物激素之间的相互作用得到了我们的累积数据的支持,这也为理解吉吉拉塔的ARF机制和抗旱提供了理论基础。