RESULTS: In this study, we identified the molecular interaction between dKeap1 and lamin Dm0, the Drosophila B-type lamin responsible for the architecture of nuclear lamina and chromatin. Ectopic expression of dKeap1 led to an ectopic localization of lamin to the intra-nuclear area, corelated with the spreading of the heterochromatin marker H3K9me2 into euchromatin regions. Additionally, mis-regulated dKeap1 disrupted the morphology of the nuclear lamina. Knocking down of dKeap1 partially rescued the lethality induced by lamin overexpression, suggesting their genetic interaction during development.
CONCLUSIONS: The discovered dKeap1-lamin interaction suggests a novel role for the Keap1 oxidative/xenobiotic response factor in regulating chromatin architecture.
结果:在这项研究中,我们确定了dKeap1和LaminDm0之间的分子相互作用,Dm0是负责核层和染色质结构的果蝇B型层蛋白。dKeap1的异位表达导致层粘连蛋白异位定位到核内区域,与异染色质标记H3K9me2扩散到常染色质区域相关。此外,错误调节的dKeap1破坏了核层的形态。dKeap1的敲除部分挽救了由lamin过度表达引起的致死率,表明它们在发育过程中的遗传相互作用。
结论:发现的dKeap1-lamin相互作用提示了Keap1氧化/异种生物反应因子在调节染色质结构方面的新作用。