Abstract：
Parathyroid hormone (PTH) interacts with components of the gut microbiota to exert its bone-regulating effects. This study aimed to investigate the gut microbial composition in patients with primary hyperparathyroidism (PHPT). Nine patients with PHPT and nine age-sex and body mass index-matched healthy controls were included. Gut microbial composition was assessed using 16S rRNA gene amplicon sequencing in both groups at baseline and 1 month after parathyroidectomy in the PHPT group. Data were imported into QIIME-2 and both QIIME-2 and R packages were used for microbiome analysis. Alpha and beta diversities were similar between the groups and remained unchanged after parathyroidectomy. The relative abundance of Subdoligranulum was significantly higher, whereas Ruminococcus, Alloprevotella, Phascolarctobacterium, and Clostridium sensu stricto_1 were significantly lower in PHPT than in controls (p < 0.001). After parathyroidectomy, the relative abundance of Subdoligranulum decreased, and Ruminococcus and Alloprevotella increased (p < 0.001). The PHPT group had lower total femoral and lumbar bone mineral density (BMD) than the controls (p < 0.05). At baseline, Alloprevotella abundance was positively correlated with serum phosphorus and Subdoligranulum was positively correlated with total lumbar BMD. Clostridium sensu stricto_1 was negatively correlated with serum calcium and positively correlated with femoral neck BMD. Postoperatively, Alloprevotella was positively correlated with baseline serum phosphorus and Phascolarctobacterium was positively correlated with distal radius BMD. This study demonstrated that the diversity of the gut microbiome was altered, possibly in response to electrolyte changes in PHPT, both before and after parathyroidectomy.
摘要：
甲状旁腺激素（PTH）与肠道微生物群的成分相互作用，发挥其骨调节作用。本研究旨在探讨原发性甲状旁腺功能亢进(PHPT)患者的肠道微生物组成。包括9名PHPT患者和9名年龄性别和体重指数匹配的健康对照。在PHPT组中,在基线和甲状旁腺切除术后1个月,使用16SrRNA基因扩增子测序评估两组的肠道微生物组成。将数据导入到QIIME-2中，并将QIIME-2和R包用于微生物组分析。两组之间的α和β多样性相似，在甲状旁腺切除术后保持不变。下颗粒的相对丰度明显较高，而Ruminococus,Alloprevotella,PHPT中的结核分枝杆菌和梭状芽胞杆菌明显低于对照组（p<0.001）。甲状旁腺切除术后,下颗粒的相对丰度下降，Ruminococus和Alloprevotella增加（p<0.001）。PHPT组的总股骨和腰椎骨密度（BMD）低于对照组（p<0.05）。在基线,Alloprevotella丰度与血清磷呈正相关，下颗粒与腰椎总骨密度呈正相关。感觉梭状芽孢杆菌1与血清钙呈负相关，与股骨颈骨密度呈正相关。术后,Alloprevotella与基线血清磷呈正相关，而根瘤菌与桡骨远端BMD呈正相关。这项研究表明，肠道微生物组的多样性发生了变化，可能是对PHPT中电解质变化的反应，甲状旁腺切除术前后。
