Abstract：
Two fluorescent probes, Y1-2 were synthesized from 2-acetonaphthone, 4-acetylbiphenyl, and phenyl hydrazine by Vilsmeier-Haack reaction and Knoevenagel condensation. Their recognition efficacies for N2H4 were tested by UV-visible absorption spectroscopy and fluorescence emission spectroscopy. The recognition mechanism were studies by density-functional theory calculations, and the effect of pH on N2H4 recognition was also studied. The results showed that the probe Y1-2 has high selectivity and a low detection limit for N2H4, and the recognition of N2H4 can be accomplished at physiological pH. The probes have had obvious aggregation-induced luminescence effect, large Stokes shift, high sensitivity, and can be successfully applied to live cell imaging.
摘要：
两个荧光探针，Y1-2由2-丙酮萘酮合成，4-乙酰联苯,和苯基肼通过Vilsmeier-Haack反应和Knoevenagel缩合。通过紫外-可见吸收光谱和荧光发射光谱测试了它们对N2H4的识别效果。通过密度泛函理论计算研究了识别机制，并研究了pH对N2H4识别的影响。结果表明，探针Y1-2对N2H4具有较高的选择性和较低的检测限，在生理pH下可以完成对N2H4的识别。探针具有明显的聚集诱导发光效应,大斯托克斯位移,高灵敏度,并可成功应用于活细胞成像。
