Abstract：
BACKGROUND: Hemolysis is a common reason for nonreporting results in biochemistry and is measured using the hemolysis index (HI), with nonreporting limits set for analytes by manufacturers.
OBJECTIVE: To verify the nonreporting HI limit for potassium, phosphate, magnesium, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), iron, CA19-9, and vitamin D on the Beckman Coulter AU5800/DxI800 analyzers.
METHODS: Hemolysate was created from EDTA-lined tubes of whole blood using an osmotic shock procedure. The hemolysate underwent serial dilutions with saline and was spiked in paired serum. The delta changes in HI and analyte concentration were measured, assessed using regression analysis, and compared against calculated reference change values.
RESULTS: A linear relationship between increasing HI and increasing analyte concentration (R2 > 0.9) was observed for potassium (y = 0.8864x), phosphate (y = 0.1079x), magnesium (y = 0.0678x), AST (y = 29.035x), and LDH (y = 350x). Increasing HI values did not have a linear effect on iron (y = -0.2544x), CA19-9 (y = 2.7019x), or vitamin D (y = 8.036x) concentrations.
CONCLUSIONS: The results from this experiment support increasing the HI nonreporting limit to 100 mg/dL for potassium; 200 mg/dL for magnesium; and 300 mg/dL for phosphate, CA19-9, and vitamin D. The iron assay is not affected by hemolysis as high as 500 mg/dL. The current HI nonreporting limit of 50 mg/dL is appropriate for LDH.
OBJECTIVE: To verify the nonreporting HI limit for potassium, phosphate, magnesium, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), iron, CA19-9, and vitamin D on the Beckman Coulter AU5800/DxI800 analyzers.
METHODS: Hemolysate was created from EDTA-lined tubes of whole blood using an osmotic shock procedure. The hemolysate underwent serial dilutions with saline and was spiked in paired serum. The delta changes in HI and analyte concentration were measured, assessed using regression analysis, and compared against calculated reference change values.
RESULTS: A linear relationship between increasing HI and increasing analyte concentration (R2 > 0.9) was observed for potassium (y = 0.8864x), phosphate (y = 0.1079x), magnesium (y = 0.0678x), AST (y = 29.035x), and LDH (y = 350x). Increasing HI values did not have a linear effect on iron (y = -0.2544x), CA19-9 (y = 2.7019x), or vitamin D (y = 8.036x) concentrations.
CONCLUSIONS: The results from this experiment support increasing the HI nonreporting limit to 100 mg/dL for potassium; 200 mg/dL for magnesium; and 300 mg/dL for phosphate, CA19-9, and vitamin D. The iron assay is not affected by hemolysis as high as 500 mg/dL. The current HI nonreporting limit of 50 mg/dL is appropriate for LDH.
摘要：
背景：溶血是生物化学中未报告结果的常见原因，使用溶血指数（HI）进行测量，制造商为分析物设定了非报告限值。
目的：为了验证钾的非报告HI限值，磷酸盐,镁，天冬氨酸转氨酶（AST），乳酸脱氢酶（LDH），铁,CA19-9和维生素D在BeckmanCoulterAU5800/DxI800分析仪上。
方法：使用渗透压休克程序从EDTA衬里的全血试管中产生溶血产物。将溶血产物用盐水连续稀释,并掺入成对血清中。测量HI和分析物浓度的δ变化，使用回归分析进行评估，并与计算的参考变化值进行比较。
结果：对于钾（y=0.8864x），观察到HI增加与分析物浓度增加之间的线性关系（R2>0.9），磷酸盐（y=0.1079x），镁（y=0.0678x），AST（y=29.035x），和LDH（y=350x）。增加HI值对铁没有线性影响（y=-0.2544x），CA19-9（y=2.7019x），或维生素D（y=8.036x）浓度。
结论：该实验的结果支持将钾的HI非报告限值提高到100mg/dL；镁为200mg/dL；磷酸盐为300mg/dL，CA19-9和维生素D。铁测定不受高达500mg/dL的溶血的影响。目前50mg/dL的HI非报告限值适用于LDH。
目的：为了验证钾的非报告HI限值，磷酸盐,镁，天冬氨酸转氨酶（AST），乳酸脱氢酶（LDH），铁,CA19-9和维生素D在BeckmanCoulterAU5800/DxI800分析仪上。
方法：使用渗透压休克程序从EDTA衬里的全血试管中产生溶血产物。将溶血产物用盐水连续稀释,并掺入成对血清中。测量HI和分析物浓度的δ变化，使用回归分析进行评估，并与计算的参考变化值进行比较。
结果：对于钾（y=0.8864x），观察到HI增加与分析物浓度增加之间的线性关系（R2>0.9），磷酸盐（y=0.1079x），镁（y=0.0678x），AST（y=29.035x），和LDH（y=350x）。增加HI值对铁没有线性影响（y=-0.2544x），CA19-9（y=2.7019x），或维生素D（y=8.036x）浓度。
结论：该实验的结果支持将钾的HI非报告限值提高到100mg/dL；镁为200mg/dL；磷酸盐为300mg/dL，CA19-9和维生素D。铁测定不受高达500mg/dL的溶血的影响。目前50mg/dL的HI非报告限值适用于LDH。
