Abstract：
In this study, the biochemical response of Phaeodactylum tricornutum to varying concentrations of inorganic selenium (Se) was investigated. It was observed that, when combined with fulvic acid, P. tricornutum exhibited enhanced uptake and biotransformation of inorganic Se, as well as increased microalgal lipid biosynthesis. Notably, when subjected to moderate (5 and 10 mg/L) and high (20 and 40 mg/L) concentrations of selenite under fulvic acid treatment, there was a discernible redirection of carbon flux towards lipogenesis and protein biosynthesis from carbohydrates. In addition, the key parameters of microalgae-based biofuels aligned with the necessary criteria outlined in biofuel regulations. Furthermore, the Se removal capabilities of P. tricornutum, assisted by fulvic acid, were coupled with the accumulation of substantial amounts of organic Se, specifically SeCys. These findings present a viable and successful approach to establish a microalgae-based system for Se uptake and biotransformation.
摘要：
在这项研究中,研究了三角Phaeodactylum对不同浓度的无机硒（Se）的生化响应。据观察，当与富里酸结合时，tricornutum表现出增强的无机硒的吸收和生物转化，以及增加微藻脂质的生物合成。值得注意的是,当在富里酸处理下经受中等（5和10mg/L）和高（20和40mg/L）浓度的亚硒酸盐时，碳通量向碳水化合物的脂肪生成和蛋白质生物合成有明显的重定向。此外,基于微藻的生物燃料的关键参数与生物燃料法规中概述的必要标准一致。此外，Tricornutum的硒去除能力，在富里酸的辅助下，再加上大量有机硒的积累，特别是SeCys.这些发现为建立基于微藻的Se吸收和生物转化系统提供了可行且成功的方法。
