Abstract：
BACKGROUND: Microorganisms colonizing the apical root canal system are conceivably the ones directly involved with the causation and maintenance of apical periodontitis.
OBJECTIVE: This article systematically reviews the reports on the microbiome occurring exclusively at the apical root canal of teeth with primary and posttreatment apical periodontitis.
METHODS: The electronic databases PubMed, Embase, Web of Science, Science Direct, and Proquest were searched up to August 2023. Clinical studies using culture and molecular microbiology methods to identify the microbial taxa present exclusively in the apical root canal segment of infected teeth with apical periodontitis were included. Studies were critically assessed using the Joanna Briggs Institute Critical Prevalence Assessment Checklist.
RESULTS: From 2277 articles initially detected, 52 were selected for full reading and 21 were eventually included in this review. Of these, molecular methods were used in 19 and culture in 2 studies. Ten studies evaluated primary infections, 8 evaluated posttreatment infections, and 3 included both. Cryopulverization of the apical root specimens was conducted in 11 studies. All studies evaluated the prevalence and diversity of bacteria, and only one also reported on fungi. Overall, the most frequent/abundant bacterial taxa found in the apical canal of primary infections were Pseudoramibacter alactolyticus, Olsenella uli, Fusobacterium species, Streptococcus species, Porphyromonas endodontalis, Prevotella species, Actinomyces species, Parvimonas micra, Treponema denticola, Synergistetes species, and an as-yet uncharacterized taxon. In posttreatment infections, the most prevalent/abundant bacterial taxa included species of Streptococcus, Enterococcus, Fusobacterium, Actinomyces, Pseudoramibacter, Pseudomonas, and Propionibacterium. At the phylum level, Firmicutes was the most represented. The average apical bacterial load ranged from 105 to 106 in primary infections and from 103 to 104 in posttreatment infections.
CONCLUSIONS: Microbial diversity in the apical part of the root canal system was examined encompassing data from both primary and posttreatment infections. Heterogeneity amongst the studies, especially in sample collection and microbial identification methods, is an important limitation that prevented a meta-analysis.
CONCLUSIONS: There is a pronounced bacterial diversity in the infected apical canal, with a high interindividual variability. Different microbiome compositions at the species/genus level are observed according to the infection type.
BACKGROUND: PROSPERO CRD42021275886.
OBJECTIVE: This article systematically reviews the reports on the microbiome occurring exclusively at the apical root canal of teeth with primary and posttreatment apical periodontitis.
METHODS: The electronic databases PubMed, Embase, Web of Science, Science Direct, and Proquest were searched up to August 2023. Clinical studies using culture and molecular microbiology methods to identify the microbial taxa present exclusively in the apical root canal segment of infected teeth with apical periodontitis were included. Studies were critically assessed using the Joanna Briggs Institute Critical Prevalence Assessment Checklist.
RESULTS: From 2277 articles initially detected, 52 were selected for full reading and 21 were eventually included in this review. Of these, molecular methods were used in 19 and culture in 2 studies. Ten studies evaluated primary infections, 8 evaluated posttreatment infections, and 3 included both. Cryopulverization of the apical root specimens was conducted in 11 studies. All studies evaluated the prevalence and diversity of bacteria, and only one also reported on fungi. Overall, the most frequent/abundant bacterial taxa found in the apical canal of primary infections were Pseudoramibacter alactolyticus, Olsenella uli, Fusobacterium species, Streptococcus species, Porphyromonas endodontalis, Prevotella species, Actinomyces species, Parvimonas micra, Treponema denticola, Synergistetes species, and an as-yet uncharacterized taxon. In posttreatment infections, the most prevalent/abundant bacterial taxa included species of Streptococcus, Enterococcus, Fusobacterium, Actinomyces, Pseudoramibacter, Pseudomonas, and Propionibacterium. At the phylum level, Firmicutes was the most represented. The average apical bacterial load ranged from 105 to 106 in primary infections and from 103 to 104 in posttreatment infections.
CONCLUSIONS: Microbial diversity in the apical part of the root canal system was examined encompassing data from both primary and posttreatment infections. Heterogeneity amongst the studies, especially in sample collection and microbial identification methods, is an important limitation that prevented a meta-analysis.
CONCLUSIONS: There is a pronounced bacterial diversity in the infected apical canal, with a high interindividual variability. Different microbiome compositions at the species/genus level are observed according to the infection type.
BACKGROUND: PROSPERO CRD42021275886.
摘要：
背景：定植于根尖根管系统的微生物可能是与根尖牙周炎的病因和维持直接相关的微生物。
目的：本文系统综述了根尖周炎患者根尖根管发生的微生物组的报道。
方法：电子数据库PubMed，Embase,WebofScience,科学直接，和Proquest被搜索到2023年8月。包括使用培养和分子微生物学方法进行的临床研究，以鉴定仅存在于根尖周炎感染牙齿的根尖根管段中的微生物类群。使用JoannaBriggs研究所关键患病率评估清单对研究进行了严格评估。
结果：从最初检测到的2277篇文章中，选择了52个进行全面阅读，最终将21个纳入本综述。其中,在19项研究中使用了分子方法，在2项研究中使用了培养方法。十项研究评估了原发性感染，8评估治疗后感染，3包括两者。在11项研究中对根尖标本进行了冷冻处理。所有研究都评估了细菌的患病率和多样性，只有一个还报道了真菌。总的来说,在原发感染的根尖管中发现的最常见/最丰富的细菌类群是假单胞菌，Olsenellauli,梭杆菌属物种，链球菌种，根管卟啉单胞菌,Prevotella物种，放线菌物种，Parvimonasmicra,Denticola密螺旋体,协同剂物种，和一个尚未表征的分类单元。在治疗后感染中，最普遍/丰富的细菌分类群包括链球菌，肠球菌,梭杆菌,放线菌,假单胞菌,假单胞菌,和丙酸杆菌。在门一级，Firmicutes代表最多。原发性感染的平均根尖细菌负荷为105至106，治疗后感染的平均根尖细菌负荷为103至104。
结论：研究了根管系统根尖部分的微生物多样性，包括来自原发和治疗后感染的数据。研究之间的异质性，特别是在样品收集和微生物鉴定方法中，是阻止荟萃分析的重要限制。
结论：感染的根尖管有明显的细菌多样性，具有很高的个体差异。根据感染类型，在物种/属水平观察到不同的微生物组组成。
背景：PROSPEROCRD42021275886。
目的：本文系统综述了根尖周炎患者根尖根管发生的微生物组的报道。
方法：电子数据库PubMed，Embase,WebofScience,科学直接，和Proquest被搜索到2023年8月。包括使用培养和分子微生物学方法进行的临床研究，以鉴定仅存在于根尖周炎感染牙齿的根尖根管段中的微生物类群。使用JoannaBriggs研究所关键患病率评估清单对研究进行了严格评估。
结果：从最初检测到的2277篇文章中，选择了52个进行全面阅读，最终将21个纳入本综述。其中,在19项研究中使用了分子方法，在2项研究中使用了培养方法。十项研究评估了原发性感染，8评估治疗后感染，3包括两者。在11项研究中对根尖标本进行了冷冻处理。所有研究都评估了细菌的患病率和多样性，只有一个还报道了真菌。总的来说,在原发感染的根尖管中发现的最常见/最丰富的细菌类群是假单胞菌，Olsenellauli,梭杆菌属物种，链球菌种，根管卟啉单胞菌,Prevotella物种，放线菌物种，Parvimonasmicra,Denticola密螺旋体,协同剂物种，和一个尚未表征的分类单元。在治疗后感染中，最普遍/丰富的细菌分类群包括链球菌，肠球菌,梭杆菌,放线菌,假单胞菌,假单胞菌,和丙酸杆菌。在门一级，Firmicutes代表最多。原发性感染的平均根尖细菌负荷为105至106，治疗后感染的平均根尖细菌负荷为103至104。
结论：研究了根管系统根尖部分的微生物多样性，包括来自原发和治疗后感染的数据。研究之间的异质性，特别是在样品收集和微生物鉴定方法中，是阻止荟萃分析的重要限制。
结论：感染的根尖管有明显的细菌多样性，具有很高的个体差异。根据感染类型，在物种/属水平观察到不同的微生物组组成。
背景：PROSPEROCRD42021275886。
