PDF(Pubmed)
Abstract：
Leishmaniasis is a vector-borne disease caused by the protozoan parasite of Leishmania genus and is a complex disease affecting mostly tropical regions of the world. Unfortunately, despite the extensive effort made, there is no vaccine available for human use. Undoubtedly, a comprehensive understanding of the host-vector-parasite interaction is substantial for developing an effective prophylactic vaccine. Recently the role of sandfly saliva on disease progression has been uncovered which can make a substantial contribution in vaccine design. In this review we try to focus on the strategies that most probably meet the prerequisites of vaccine development (based on the current understandings) including live attenuated/non-pathogenic and subunit DNA vaccines. Innovative approaches such as reverse genetics, CRISP/R-Cas9 and antibiotic-free selection are now available to promisingly compensate for intrinsic drawbacks associated with these platforms. Our main goal is to call more attention toward the prerequisites of effective vaccine development while controlling the disease outspread is a substantial need.
摘要：
利什曼病是由利什曼原虫属的原生动物寄生虫引起的媒介传播疾病，是一种影响世界上大部分热带地区的复杂疾病。不幸的是,尽管付出了巨大的努力，没有可供人类使用的疫苗。毫无疑问,全面了解宿主-载体-寄生虫的相互作用对于开发有效的预防性疫苗具有重要意义.最近，已经发现了沙蝇唾液在疾病进展中的作用，这可以在疫苗设计中做出重大贡献。在这篇综述中，我们试图关注最有可能满足疫苗开发先决条件的策略（基于当前的理解），包括减毒活疫苗/非致病性疫苗和亚单位DNA疫苗。创新方法，如反向遗传学，CRISP/R-Cas9和无抗生素选择现在可以有希望地补偿与这些平台相关的内在缺点。我们的主要目标是呼吁更多关注有效疫苗开发的先决条件，同时控制疾病的蔓延是一个巨大的需求。
