PDF(Pubmed)
Abstract：
UNASSIGNED: Clostridium perfringens (CP) is an emerging anaerobic pathogen that can aggravate severe fatal infections in different hosts and livestock.
UNASSIGNED: This paper was designed to monitor the antibacterial efficacy of Moringa oleifera (M. oleifera) plant against different CP isolates of variant toxin genotypes comparing that with commercial antibiotics in the veterinary field.
UNASSIGNED: A total of 200 examined fecal, intestinal, and liver samples from cattle, sheep, and goats were investigated bacteriologically and biochemically for CP. Then, the isolates were examined by polymerase chain reaction (PCR) for toxin gene typing. Thereafter, the antimicrobial susceptibility testing as well as the antibacterial efficacy of M. oleifera were evaluated and statistically analyzed against recovered isolates.
UNASSIGNED: The prevalence rate of CP was 51% (102/200); of which 54.5% was from cattle, 50% from sheep, and 40% from goat. Moreover, all CP isolates were highly resistant to tetracycline and lincomycin drugs; meanwhile, they were of the least resistance against ciprofloxacin (8.3%-16.7%), cefotaxime (16.7%-25%), and gentamycin (26.7%-33.3%). For M. oleifera, high antibacterial efficacy with greater inhibition zones of the plant was recorded with its oil (20-24 mm) and ethanolic extracts (16-20 mm) against CP than the aqueous extract (≤ 10 mm). A good correlation was stated between M. oleifera oil and toxin type of CP isolates particularly type A followed by D and B types. Interestingly, the oil and ethanolic extracts of M. oleifera gave higher antibacterial efficacy than most commercial antibiotics against the recovered isolates.
UNASSIGNED: This study highlighted the potent antibacterial properties of M. oleifera for suppressing CP isolated from farm animals; hence, more investigations on M. oleifera are suggested to support its use as a medical herbal plant substituting antibiotics hazards and resistance problems worldwide.
UNASSIGNED: This paper was designed to monitor the antibacterial efficacy of Moringa oleifera (M. oleifera) plant against different CP isolates of variant toxin genotypes comparing that with commercial antibiotics in the veterinary field.
UNASSIGNED: A total of 200 examined fecal, intestinal, and liver samples from cattle, sheep, and goats were investigated bacteriologically and biochemically for CP. Then, the isolates were examined by polymerase chain reaction (PCR) for toxin gene typing. Thereafter, the antimicrobial susceptibility testing as well as the antibacterial efficacy of M. oleifera were evaluated and statistically analyzed against recovered isolates.
UNASSIGNED: The prevalence rate of CP was 51% (102/200); of which 54.5% was from cattle, 50% from sheep, and 40% from goat. Moreover, all CP isolates were highly resistant to tetracycline and lincomycin drugs; meanwhile, they were of the least resistance against ciprofloxacin (8.3%-16.7%), cefotaxime (16.7%-25%), and gentamycin (26.7%-33.3%). For M. oleifera, high antibacterial efficacy with greater inhibition zones of the plant was recorded with its oil (20-24 mm) and ethanolic extracts (16-20 mm) against CP than the aqueous extract (≤ 10 mm). A good correlation was stated between M. oleifera oil and toxin type of CP isolates particularly type A followed by D and B types. Interestingly, the oil and ethanolic extracts of M. oleifera gave higher antibacterial efficacy than most commercial antibiotics against the recovered isolates.
UNASSIGNED: This study highlighted the potent antibacterial properties of M. oleifera for suppressing CP isolated from farm animals; hence, more investigations on M. oleifera are suggested to support its use as a medical herbal plant substituting antibiotics hazards and resistance problems worldwide.
摘要：
■产气荚膜梭菌（CP）是一种新兴的厌氧病原体，可加重不同宿主和牲畜的严重致命感染。
■本文旨在监测辣木（M.oleifera）植物针对不同CP分离株的变异毒素基因型，与兽医领域的商业抗生素相比。
■总共检查了200个粪便，肠,和牛的肝脏样本，绵羊,对山羊进行了CP的细菌学和生化研究。然后，通过聚合酶链反应（PCR）检查分离株的毒素基因分型。此后,我们对回收的分离株进行了抗菌药物敏感性试验以及油菌的抗菌功效评估和统计学分析.
■CP的患病率为51％（102/200）；其中54.5％来自牛，50%来自绵羊，40%来自山羊。此外，所有CP分离株对四环素和林可霉素药物均高度耐药；同时，他们对环丙沙星的耐药性最低（8.3％-16.7％），头孢噻肟（16.7%-25%），和庆大霉素（26.7%-33.3%）。对于油菌来说,与水提取物（≤10mm）相比，其油（20-24mm）和乙醇提取物（16-20mm）对CP具有较高的抗菌效力，对植物的抑制作用更大。油菌油与CP分离株的毒素类型之间存在良好的相关性，特别是A型，其次是D和B型。有趣的是,油菌的油和乙醇提取物对回收的分离株具有比大多数商业抗生素更高的抗菌效力。
■这项研究强调了油菌对抑制从农场动物中分离的CP的有效抗菌特性；因此，对油菌的更多研究被建议支持其作为替代抗生素危害和全球耐药性问题的医学草药植物。
■本文旨在监测辣木（M.oleifera）植物针对不同CP分离株的变异毒素基因型，与兽医领域的商业抗生素相比。
■总共检查了200个粪便，肠,和牛的肝脏样本，绵羊,对山羊进行了CP的细菌学和生化研究。然后，通过聚合酶链反应（PCR）检查分离株的毒素基因分型。此后,我们对回收的分离株进行了抗菌药物敏感性试验以及油菌的抗菌功效评估和统计学分析.
■CP的患病率为51％（102/200）；其中54.5％来自牛，50%来自绵羊，40%来自山羊。此外，所有CP分离株对四环素和林可霉素药物均高度耐药；同时，他们对环丙沙星的耐药性最低（8.3％-16.7％），头孢噻肟（16.7%-25%），和庆大霉素（26.7%-33.3%）。对于油菌来说,与水提取物（≤10mm）相比，其油（20-24mm）和乙醇提取物（16-20mm）对CP具有较高的抗菌效力，对植物的抑制作用更大。油菌油与CP分离株的毒素类型之间存在良好的相关性，特别是A型，其次是D和B型。有趣的是,油菌的油和乙醇提取物对回收的分离株具有比大多数商业抗生素更高的抗菌效力。
■这项研究强调了油菌对抑制从农场动物中分离的CP的有效抗菌特性；因此，对油菌的更多研究被建议支持其作为替代抗生素危害和全球耐药性问题的医学草药植物。
