Abstract：
The lesser mealworm, Alphitobius diaperinus, is an invasive tenebrionid beetle and a vector of pathogens. Due to the emergence of insecticide resistance and consequent outbreaks that generate significant phytosanitary and energy costs for poultry farmers, it has become a major insect pest worldwide. To better understand the molecular mechanisms behind this resistance, we studied a strain of A. diaperinus from a poultry house in Brittany that was found to be highly resistant to the β-cyfluthrin. The strain survived β-cyfluthrin exposures corresponding to more than 100 times the recommended dose. We used a comparative de novo RNA-Seq approach to explore genes expression in resistant versus sensitive strains. Our de novo transcriptomic analyses showed that responses to β-cyfluthrin likely involved a whole set of resistance mechanisms. Genes related to detoxification, metabolic resistance, cuticular hydrocarbon biosynthesis and proteolysis were found to be constitutively overexpressed in the resistant compared to the sensitive strain. Follow-up enzymatic assays confirmed that the resistant strain exhibited high basal activities for detoxification enzymes such as cytochrome P450 monooxygenase and glutathione-S-transferase. The in-depth analysis of differentially expressed genes suggests the involvement of complex regulation of signaling pathways. Detailed knowledge of these resistance mechanisms is essential for the establishment of effective pest control.
摘要：
较小的粉虫，Alphitobiusdiaperinus,是一种侵袭性的黄连虫甲虫和病原体的媒介。由于杀虫剂抗性的出现和随之而来的爆发，给家禽养殖户带来了巨大的植物检疫和能源成本，它已成为世界范围内的主要害虫。为了更好地理解这种抗性背后的分子机制，我们研究了布列塔尼一家禽舍中的一株双食A。被发现对β-氟氯氰菊酯具有高度抗性。该菌株在β-氟氯氰菊酯暴露中存活，相当于推荐剂量的100倍以上。我们使用从头比较RNA-Seq方法来探索抗性菌株与敏感菌株中的基因表达。我们的从头转录组分析表明，对β-氟氯氰菊酯的反应可能涉及一整套抗性机制。与解毒有关的基因，代谢抗性，与敏感菌株相比，发现表皮烃生物合成和蛋白水解在抗性中组成性过表达。后续酶促测定证实，抗性菌株对解毒酶如细胞色素P450单加氧酶和谷胱甘肽-S-转移酶表现出较高的基础活性。对差异表达基因的深入分析提示信号通路的复杂调控。对这些抗性机制的详细了解对于建立有效的害虫控制至关重要。
