PDF(Pubmed)
Abstract：
Toxoplasmosis is a frequent infection among the human population. The infection can cause devastating complications for the fetus during pregnancy. The present study aimed to determine the serological and molecular prevalence of the infection and molecular characterization of Toxoplasma gondii isolates among pregnant women referred to Kowsar Hospital, Urmia, Iran. In a cross-sectional study, 340 blood samples were collected from pregnant women referred to Kowsar Hospital, Urmia, Iran from May to July 2022. Anti-T. gondii IgG and IgM seropositivity were determined by enzyme-linked immunosorbent assay. PCR was carried out by targeting the GRA6 gene of the parasite on all patients\' buffy coats. Anti-T. gondii IgG and IgM antibodies were positive in two (0.6%) women, and 101 (29.7%) women had anti-T. gondii IgG and 70.3% were seronegative. PCR was positive in two IgM-positive women, and both isolates belonged to T. gondii carrying the GRA6 allele of lineage I. The risk of infection was significantly higher in women who had constant contact with cats and soil, and who were residents of rural areas. The two IgM-positive women were asymptomatic regarding acute toxoplasmosis. According to the results of the present study, the prevalence of toxoplasmosis in pregnant women in Urmia is similar to its prevalence in other areas in northwestern Iran, and despite the low prevalence of acute infection, it should not be ignored.
摘要：
弓形虫病是人群中常见的感染。感染会在怀孕期间对胎儿造成毁灭性的并发症。本研究旨在确定转诊至Kowsar医院的孕妇中弓形虫分离株的感染和分子特征的血清学和分子学患病率，Urmia,伊朗。在一项横断面研究中，从转诊至Kowsar医院的孕妇中收集了340份血液样本，Urmia,伊朗从2022年5月到7月。反T.通过酶联免疫吸附测定测定刚地IgG和IgM血清阳性。通过在所有患者的血沉棕黄层上靶向寄生虫的GRA6基因进行PCR。反T.两名(0.6%)女性的gondiiIgG和IgM抗体呈阳性,101名(29.7%)女性患有抗T.gondiiIgG和70.3%血清阴性。两名IgM阳性女性的PCR呈阳性,并且两个分离株都属于携带谱系I的GRA6等位基因的弓形虫，在经常与猫和土壤接触的女性中，感染的风险明显更高，他们是农村地区的居民。两名IgM阳性妇女因急性弓形虫病无症状。根据本研究的结果,Urmia孕妇弓形虫病的患病率与伊朗西北部其他地区的患病率相似，尽管急性感染的患病率较低，它不应该被忽视。
