Abstract：
Free-living amoebae (FLA) of the genus Acanthamoeba are ubiquitous and amphizoic protozoa that colonize aquatic and terrestrial habitats and can serve as reservoirs for other microorganisms. They are considered econoses that can cause severe and rare pathologies. Due to limited epidemiological data available, the objective of this study was to investigate the presence of Acanthamoeba in coastal wetlands of the southeast of Buenos Aires province and evaluate their association with bacteriological and environmental variables. From February 2021 to July 2022, 22 seawater samples were collected at different points along the coast of the city of Mar del Plata (Buenos Aires, Argentina). Environmental parameters were determined and physicochemical and bacteriological studies, morphological identification, cultures and molecular typification were conducted. Regardless of the environmental and bacteriological variables, the presence of Acanthamoeba spp. was molecularly confirmed in 54.54% of the samples, being the first report of these protozoa in seawater in Argentina.
摘要：
棘阿米巴属的自由生活变形虫（FLA）是无处不在的两栖动物原生动物，它们在水生和陆生栖息地定居，并可以作为其他微生物的水库。它们被认为是可引起严重和罕见病变的生态病。由于流行病学数据有限，这项研究的目的是调查布宜诺斯艾利斯省东南部沿海湿地中棘阿米巴的存在，并评估其与细菌学和环境变量的关系。从2021年2月到2022年7月，在马德普拉塔市沿海的不同地点收集了22个海水样本（布宜诺斯艾利斯，阿根廷)。确定了环境参数，并进行了物理化学和细菌学研究，形态学鉴定，进行了培养和分子分型。无论环境和细菌学变量如何，棘阿米巴的存在。在54.54%的样品中得到分子证实，是阿根廷海水中这些原生动物的第一份报告。
