Abstract：
Hereunder, we pioneered the synthesis of Copper Oxide nanoparticles (CuO NPs) utilizing Tragacanth gum (TG). The NPs were characterized using advanced techniques and assessed for different pharmaceutical and environmental perspectives. The successful formation of a colloidal NPs solution was confirmed by the appearance of a distinct black color and a distinct peak at 260 nm in UV-Visible spectrophotometry. The FTIR analysis unveiled a spectrum of functional groups responsible for the reduction and stabilization of CuO NPs. Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) revealed size of NPs as 36.24 nm and 28 ± 04 nm respectively. Energy Dispersive X-ray (EDX) Analysis indicated weight percentages of 70.38 % for Cu and 18.88 % for O, with corresponding atomic percentages. The X-ray Diffraction (XRD) analysis revealed the orthorhombic crystal structure of the prepared CuO NPs. Antimicrobial assessments through disc-diffusion assays demonstrated significant zones of inhibition (ZOI) against gram-positive bacterial strains (Bacillus Halodurans and Micrococcus leutus) and a gram-negative bacterial strain (E. coli). Against the fungal strain Aspergillus niger, a ZOI of 18.5 ± 0.31 mm was observed. The NPs exhibited remarkable antioxidant potential determined through 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and H2O2 scavenging assays. At a concentration of 3 mg/mL, the NPs demonstrated biofilm inhibition rates of 96 %, 90 %, 89.60 %, and 72.10 % against Micrococcus luteus, Bacillus halodurans, MRSA and E.coli respectively. Furthermore, the CuO NPs showed a high photocatalytic potential towards the degradation of safranin dye under sunlight irradiation. In conclusion, the findings underline the promising multifunctional properties of TG-based CuO NPs for different practical applications.
摘要：
以下,我们率先使用Tragacanth胶（TG）合成氧化铜纳米颗粒（CuONPs）。使用先进技术对NP进行了表征，并针对不同的制药和环境观点进行了评估。通过在UV-可见光分光光度法中明显的黑色和在260nm处的明显峰的出现证实了胶体NP溶液的成功形成。FTIR分析揭示了负责CuONP的还原和稳定的官能团的光谱。动态光散射(DLS)和透射电子显微镜(TEM)显示NP的尺寸分别为36.24nm和28±04nm。能量色散X射线（EDX）分析表明，Cu的重量百分比为70.38％，O的重量百分比为18.88％，具有相应的原子百分比。X射线衍射（XRD）分析揭示了制备的CuONPs的正交晶体结构。通过圆盘扩散测定进行的抗微生物评估表明，对革兰氏阳性细菌菌株（卤水芽孢杆菌和亮斑微球菌）和革兰氏阴性细菌菌株（E.大肠杆菌）。针对真菌菌株黑曲霉，观察到18.5±0.31mm的ZOI。通过2,2-二苯基-1-吡啶酰肼（DPPH）和H2O2清除试验确定，NP具有显着的抗氧化潜力。在3mg/mL的浓度下，NPs表现出96%的生物膜抑制率，90%,89.60%,和72.10%的黄体微球菌，盐芽孢杆菌,分别为MRSA和大肠杆菌。此外，在阳光照射下，CuONPs对藏红染料的降解具有很高的光催化潜力。总之,这些发现强调了TG基CuONPs在不同实际应用中的有前途的多功能性能。
