PDF(Pubmed)
Abstract：
In this study, a novel genotype of genogroup X (GX) sapovirus (family Caliciviridae) was detected in the small intestinal contents of a golden jackal (Canis aureus) in Hungary and characterised by viral metagenomics and next-generation sequencing techniques. The complete genome of the detected strain, GX/Dömsöd/DOCA-11/2020/HUN (PP105600), is 7,128 nt in length. The ORF1- and ORF2-encoded viral proteins (NSP, VP1, and VP2) have 98%, 95%, and 88% amino acid sequence identity to the corresponding proteins of genogroup GX sapoviruses from domestic pigs, but the nucleic acid sequence identity values for their genes are significantly lower (83%, 77%, and 68%). During an RT-PCR-based epidemiological investigation of additional jackal and swine samples, no other GX strains were detected, but a GXI sapovirus strain, GXI/Tótfalu/WBTF-10/2012/HUN (PP105601), was identified in a faecal sample from a wild boar (Sus scrofa). We report the detection of members of two likely underdiagnosed groups of sapoviruses (GX and GXI) in a golden jackal and, serendipitously, in a wild boar in Europe.
摘要：
在这项研究中,在匈牙利金jack狼(Canisaureus)的小肠内容物中检测到一种新的基因组X(GX)索病毒(Caliciviviridae家族)基因型,并通过病毒宏基因组学和下一代测序技术进行表征.检测到的菌株的完整基因组，GX/Dömsöd/DOCA-11/2020/HUN(PP105600),长度为7,128nt。ORF1和ORF2编码的病毒蛋白（NSP，VP1和VP2)有98%，95%,与来自家猪的基因群GXsapovirus的相应蛋白具有88%的氨基酸序列同一性，但是其基因的核酸序列同一性值显着降低（83％，77%,和68%）。在对其他jack狼和猪样本进行基于RT-PCR的流行病学调查中，没有检测到其他GX菌株，而是一种GXI道膜病毒株，GXI/Tótfalu/WBTF-10/2012/HUN(PP105601),在野猪（Susscrofa）的粪便样本中鉴定。我们报告了在金jack狼中检测到两个可能未诊断的sapovirus病毒组（GX和GXI）的成员，偶然,在欧洲的野猪身上。
