Abstract：
A highly divergent bovine calicivirus was identified in an Indian calf with enteritis. The whole genome of this virus was sequenced, revealing distinct amino acid motifs in the polyprotein encoded by open reading frame 1 (ORF1) that are unique to caliciviruses. Phylogenetic analysis showed that it was related to members of the genus Nebovirus of the family Caliciviridae. Although it showed only 33.7-34.2% sequence identity in the VP1 protein to the nebovirus prototype strains, it showed 90.6% identity in VP1 to Kirklareli virus, a nebovirus detected in calves with enteritis in Turkey in 2012. An in-house-designed and optimized reverse transcription polymerase chain reaction (RT-PCR) assay was used to screen 120 archived bovine diarrhoeic fecal samples, 40 each from the Indian states of Uttar Pradesh, Haryana, and Himachal Pradesh, revealing frequent circulation of these divergent caliciviruses in the bovine population, with an overall positivity rate of 64.17% (77/120). This underscores the importance of conducting a comprehensive investigation of the prevalence of these divergent caliciviruses and assessing their associations with other pathogens responsible for enteritis in India.
摘要：
在患有肠炎的印度小牛中发现了高度分歧的牛杯状病毒。对这种病毒的全基因组进行了测序,揭示了由开放阅读框1（ORF1）编码的多蛋白中独特的氨基酸基序。系统发育分析表明，它与杯形病毒科的Nebovirus属成员有关。尽管它在VP1蛋白中与nebovirus原型毒株仅显示出33.7-34.2％的序列同一性，它在Kirklareli病毒的VP1中显示出90.6%的同一性，2012年在土耳其的肠炎小牛中检测到一种病毒。内部设计和优化的逆转录聚合酶链反应（RT-PCR）测定法用于筛选120个存档的牛腹泻粪便样品，来自北方邦的印度各邦的40人，哈里亚纳邦,和喜马al尔邦,揭示了这些不同的杯状病毒在牛群中的频繁循环，总体阳性率为64.17%(77/120)。这强调了对这些不同的杯状病毒的流行进行全面调查并评估其与印度引起肠炎的其他病原体的关联的重要性。
