Abstract：
In situ hybridization (ISH) is an important technique for identifying gene expression at the cellular level in various organs, including brain slices. This approach hybridizes nucleic acid probes to cellular mRNA, allowing the detection of transcriptional products. Recent advances have enabled RNA preservation in formalin-fixed paraffin-embedded (FFPE) samples, making ISH applicable to brain tumor diagnosis and research. Here, we provide a concise overview of the standard application of chromogenic ISH in neuroscience research and neuropathology practice using FFPE blocks of brain slice sections.
摘要：
原位杂交(ISH)是在细胞水平上识别各种器官中基因表达的重要技术。包括脑切片.这种方法将核酸探针与细胞mRNA杂交，允许检测转录产物。最近的进展使福尔马林固定石蜡包埋（FFPE）样品中的RNA保存成为可能，使ISH适用于脑肿瘤的诊断和研究。这里,我们简要概述了显色ISH在神经科学研究和神经病理学实践中使用脑切片的FFPE块的标准应用。
