Abstract：
Human embryonic stem cells (hESCs) resemble the pluripotent epiblast cells found in the early postimplantation human embryo and represent the \"primed\" state of pluripotency. One factor that helps primed pluripotent cells retain pluripotency and prepare genes for differentiation is the transcription factor TCF7L1, a member of a small family of proteins known as T cell factors/Lymphoid enhancer factors (TCF/LEF) that act as downstream components of the WNT signaling pathway. Transcriptional output of the WNT pathway is regulated, in part, by the activity of TCF/LEFs in conjunction with another component of the WNT pathway, β-CATENIN. Because TCF7L1 plays an important role in regulating pluripotency, we began to characterize the protein complex associated with TCF7L1 when bound to chromatin in hESCs using rapid immunoprecipitation of endogenous proteins (RIME). Data are available via ProteomeXchange with identifier PXD047582. These data identify known and new partners of TCF7L1 on chromatin and provide novel insights into how TCF7L1 and pluripotency itself might be regulated.
摘要：
人类胚胎干细胞（hESC）类似于在早期移植后人类胚胎中发现的多能外胚层细胞，代表多能性的“启动”状态。有助于引发的多能细胞保持多能性并制备用于分化的基因的一个因子是转录因子TCF7L1,其是被称为T细胞因子/淋巴增强因子(TCF/LEF)的小蛋白家族的成员,其充当WNT信号传导途径的下游组分。WNT途径的转录输出是受调控的，在某种程度上,通过TCF/LEF的活性与WNT途径的另一个组成部分相结合，β-catenin.因为TCF7L1在调节多能性中起着重要作用，我们开始使用内源性蛋白的快速免疫沉淀(RIME)表征与hESC中染色质结合时与TCF7L1相关的蛋白复合物.数据可通过ProteomeXchange获得,标识符为PXD047582。这些数据确定了TCF7L1在染色质上的已知和新的伴侣，并提供了关于TCF7L1和多能性本身如何被调节的新见解。
