PDF(Pubmed)
Abstract：
UNASSIGNED: Sepsis poses a significant threat to human life, rendering it a burdensome medical disease. Despite significant advancements, the current state of medical science still lacks a viable and efficacious cure. Costunolide (COST) is a multifaceted sesquiterpene lactone that exhibits a range of actions, including anti-inflammatory and antioxidant properties. We investigated the potential impacts of COST on a rat sepsis model caused by cecal ligation and puncture (CLP).
UNASSIGNED: We created an experimental rat model with the following groups: SHAM, CLP, CLP+low dose COST, and CLP+high dose COST. Blood, kidney, and lung samples were collected. Inflammatory mediators such as interleukin-1beta (IL-1β), IL-6, tumor necrosis factor-alpha (TNF- α), and nuclear factor kappa-B (NF-κB) were investigated. In addition, we assessed oxidative stress by measuring 8-Hydroxydeoxyguanosine (8-OHdG) immunopositivity, MDA levels, glutathione (GSH), and superoxide dismutase (SOD) activity. Histopathological and immunohistochemical examinations backed up our findings.
UNASSIGNED: Compared to the CLP group, the COST group showed a reduction in inflammatory and oxidative stress indicators. The expression of inflammatory mediators was suppressed by COST, and histological examinations revealed improvements in kidney and lung tissues in the treatment groups.
UNASSIGNED: Our study highlights the preventive effects of COST against CLP-induced sepsis-related injury. Considering its beneficial effects against many diseases, COST is worthy as to be evaluated against sepsis.
UNASSIGNED: We created an experimental rat model with the following groups: SHAM, CLP, CLP+low dose COST, and CLP+high dose COST. Blood, kidney, and lung samples were collected. Inflammatory mediators such as interleukin-1beta (IL-1β), IL-6, tumor necrosis factor-alpha (TNF- α), and nuclear factor kappa-B (NF-κB) were investigated. In addition, we assessed oxidative stress by measuring 8-Hydroxydeoxyguanosine (8-OHdG) immunopositivity, MDA levels, glutathione (GSH), and superoxide dismutase (SOD) activity. Histopathological and immunohistochemical examinations backed up our findings.
UNASSIGNED: Compared to the CLP group, the COST group showed a reduction in inflammatory and oxidative stress indicators. The expression of inflammatory mediators was suppressed by COST, and histological examinations revealed improvements in kidney and lung tissues in the treatment groups.
UNASSIGNED: Our study highlights the preventive effects of COST against CLP-induced sepsis-related injury. Considering its beneficial effects against many diseases, COST is worthy as to be evaluated against sepsis.
摘要：
■脓毒症对人类生命构成重大威胁，使它成为一种繁重的医学疾病。尽管取得了重大进展，医学科学的现状仍然缺乏可行和有效的治疗方法。Costunolide（COST）是一种多方面的倍半萜内酯，表现出一系列的作用，包括抗炎和抗氧化特性。我们研究了COST对盲肠结扎和穿孔（CLP）引起的大鼠脓毒症模型的潜在影响。
■我们创建了一个实验大鼠模型，其中包括以下组：SHAM，CLP,CLP+低剂量COST,和CLP+高剂量成本。血，肾,并收集肺样本。炎症介质，如白细胞介素-1β（IL-1β），IL-6，肿瘤坏死因子-α（TNF-α），研究了核因子κB（NF-κB）。此外,我们通过测量8-羟基脱氧鸟苷（8-OHdG）免疫阳性来评估氧化应激，MDA水平,谷胱甘肽(GSH),超氧化物歧化酶(SOD)活性。组织病理学和免疫组织化学检查支持了我们的发现。
■与CLP组相比，COST组显示炎症和氧化应激指标降低.炎症介质的表达被COST抑制，组织学检查显示治疗组肾脏和肺组织改善。
■我们的研究强调了COST对CLP诱导的脓毒症相关损伤的预防作用。考虑到它对许多疾病的有益作用，COST值得对脓毒症进行评估。
■我们创建了一个实验大鼠模型，其中包括以下组：SHAM，CLP,CLP+低剂量COST,和CLP+高剂量成本。血，肾,并收集肺样本。炎症介质，如白细胞介素-1β（IL-1β），IL-6，肿瘤坏死因子-α（TNF-α），研究了核因子κB（NF-κB）。此外,我们通过测量8-羟基脱氧鸟苷（8-OHdG）免疫阳性来评估氧化应激，MDA水平,谷胱甘肽(GSH),超氧化物歧化酶(SOD)活性。组织病理学和免疫组织化学检查支持了我们的发现。
■与CLP组相比，COST组显示炎症和氧化应激指标降低.炎症介质的表达被COST抑制，组织学检查显示治疗组肾脏和肺组织改善。
■我们的研究强调了COST对CLP诱导的脓毒症相关损伤的预防作用。考虑到它对许多疾病的有益作用，COST值得对脓毒症进行评估。
