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Abstract：
Background This study investigates Merremia emarginata\'s curative effectiveness against colon cancer cells. M. emarginata, often known as Elika jemudu, is a Convolvulaceae family plant. The inhibitory ability of anticancer herbal extracts against cancer cell growth and mediators is tested. Aim This study aims to evaluate the potent anticancer activity of M. emarginata against colon cancer cell line (HT-29). Materials and methods M. emarginata leaves were gathered and processed using solvent extraction. Anticancer activity on colon cancer cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and cysteine aspartic acid protease-3 (caspase 3), B-cell lymphoma 2 (Bcl-2), and B-cell lymphoma-extra large (Bcl-xL) mRNA expressions. The data was reported as the mean ± SD of three separate experiments done in triplicate. The statistical analysis was carried out using one-way analysis of variance (ANOVA), with a p-value less than 0.05 indicating statistical significance. Results The cell viability test showed a gradual decrease in cell growth and proliferation as the concentration increased. The ethanolic extract of M. emarginata was found to be cytotoxic against colon caller cell lines. The extract was able to induce apoptosis of cancer as revealed by Bcl-2, Bcl-xL, and caspase-3 (p<0.05 and p<0.001) signaling pathways. Conclusion M. emarginata extracts showed good anticancer activity against colon cancer cell lines. Further work is required to establish and identify the chemical constituent responsible for its anticancer activity.
摘要：
背景本研究探讨了黑质对结肠癌细胞的疗效。M.emarginata,通常被称为Elikajemudu，是一种变异科植物。测试了抗癌草药提取物对癌细胞生长和介质的抑制能力。目的本研究旨在评估M.emarginata对结肠癌细胞系（HT-29）的有效抗癌活性。材料和方法收集emarginata叶并使用溶剂萃取进行处理。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）测试和半胱氨酸天冬氨酸蛋白酶3（caspase3）测量对结肠癌细胞的抗癌活性，B细胞淋巴瘤2（Bcl-2），B细胞淋巴瘤-超大(Bcl-xL)mRNA表达。数据报告为一式三份进行的三个独立实验的平均值±SD。使用单向方差分析(ANOVA)进行统计分析，p值小于0.05，表明有统计学意义。结果细胞活力试验显示，随着浓度的增加，细胞生长和增殖逐渐减少。发现M.emarginata的乙醇提取物对结肠呼叫者细胞系具有细胞毒性。如Bcl-2，Bcl-xL所示，提取物能够诱导癌细胞凋亡，和caspase-3(p<0.05和p<0.001)信号通路。结论emarginata提取物对结肠癌细胞株具有良好的抗癌活性。需要进一步的工作来建立和鉴定负责其抗癌活性的化学成分。
