PDF(Pubmed)
Abstract：
Secreted frizzled-related proteins (SFRPs) are involved in the development of various types of cancer and function by suppressing the Wnt signaling pathway. To elucidate the clinical implications of SFRPs in uterine sarcoma, SFRP expression levels and their effects on uterine leiomyosarcoma cells were examined. Immunostaining for SFRP4 was performed on uterine smooth muscle, uterine fibroid and uterine leiomyosarcoma tissues. Additionally, the effects of SFRP4 administration on cell viability, migration and adhesion were evaluated in uterine leiomyosarcoma SKN cells using the WST-1 assay (Roche Diagnostics) and the CytoSelect™ 24-well Cell Migration Assay Kit and the CytoSelect™ 48-well Cell Adhesion Assay Kit. The expression levels of SFRP4 in uterine leiomyosarcoma tissues were lower than those in normal smooth muscle and uterine fibroid tissues. In addition, SFRP4 suppressed the viability and migration, and increased the adhesion ability of uterine leiomyosarcoma cells compared with in the control group. In conclusion, SFRP4 may suppress the viability and migration, and enhance the adhesion of sarcoma cells. These results suggested that SFRP4 could be considered as a novel therapeutic target for uterine sarcoma.
摘要：
分泌型卷曲相关蛋白（SFRP）参与各种类型癌症的发展，并通过抑制Wnt信号通路发挥功能。为了阐明SFRP在子宫肉瘤中的临床意义，检查SFRP表达水平及其对子宫平滑肌肉瘤细胞的影响。对子宫平滑肌进行SFRP4免疫染色，子宫肌瘤和子宫平滑肌肉瘤组织。此外,SFRP4给药对细胞活力的影响，使用WST-1测定(Roche诊断)和CytoSelect™24孔细胞迁移测定试剂盒和CytoSelect™48孔细胞粘附测定试剂盒评估子宫平滑肌肉瘤SKN细胞的迁移和粘附。SFRP4在子宫平滑肌肉瘤组织中的表达水平低于正常平滑肌和子宫肌瘤组织。此外,SFRP4抑制了生存能力和迁移，与对照组相比，子宫平滑肌肉瘤细胞的粘附能力增强。总之,SFRP4可以抑制生存能力和迁移，增强肉瘤细胞的粘附。这些结果表明SFRP4可以被认为是子宫肉瘤的新治疗靶标。
