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Abstract：
This study aims to investigate the gene expression of sperm-borne phospholipase C zeta (PLCζ), WW domain-binding protein 2N-Terminal Like (WBP2NL), and Tumor necrosis factor (TNF-α), as a negative control, in spermatozoa and their relationship with fertility and seminal quality in stallions. Ejaculates from 40 Criollo stallions were used, whose fertility was assessed on the basis of their pregnancy rate per cycle in at least two breeding seasons. Pregnancy rates ranged from 20% to 90% and were used to divide the stallions into two groups: High rates (≥ 50%) (n = 25), and Low rates (< 50%) (n = 15). A computer-assisted sperm analysis system - (CASA) analyzed semen after collection. Also were evaluated the physical and functional integrity of the plasmatic membrane and sperm morphology alterations. All stallions expressed PLCζ, WBP2NL, and TNF-α. PLCζ positively correlates with conception rate, total motility (TM), progressive motility (PM), plasmatic membrane functionality, and integrity. A simple linear regression was detected between pregnancy rate and PLCζ expression (P = 0.003), TM (P < 0.001) and PM (P < 0.001). PLCζ gene expression was higher (P = 0,012) in the High rates group than in the Low group. WBP2NL and TNF-α did not correlate with seminal quality and stallion\'s fertility. It was concluded that PLCζ gene expression in the spermatozoa might be used as a biomarker of fertility and seminal quality in stallions. Parameters of sperm kinetics also showed, positive correlation between TM, PM and pregnancy rate.
摘要：
本研究旨在探讨精子携带磷脂酶Cζ(PLCζ)的基因表达,WW结构域结合蛋白2N末端样(WBP2NL),和肿瘤坏死因子（TNF-α），作为阴性对照，精子及其与种马生育能力和精液质量的关系。使用了40匹克里奥尔种马的射精，根据至少两个繁殖季节每个周期的怀孕率评估其生育能力。妊娠率从20％到90％不等，并用于将种马分为两组：高率（≥50％）（n=25），和低比率(<50%)(n=15)。计算机辅助精子分析系统-（CASA）分析收集后的精液。还评估了质膜和精子形态改变的物理和功能完整性。所有种马都表达PLCζ，WBP2NL,和TNF-α。PLCζ与受孕率呈正相关，总运动性(TM)，渐进运动（PM），质膜功能，和正直。检测到妊娠率与PLCζ表达之间的简单线性回归（P=0.003）。TM(P<0.001)和PM(P<0.001)。高速率组的PLCζ基因表达高于低速率组（P=0.012）。WBP2NL和TNF-α与精液质量和种马的生育力无关。结论是，精子中PLCζ基因的表达可能被用作种马生育力和精液质量的生物标志物。精子动力学参数也显示，TM之间呈正相关，PM和怀孕率。
