PDF(Pubmed)
Abstract：
Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development. In this study, the effect of plant growth regulators together with basal microalgal cultivation medium on biomass, total lipid, and EPA production was studied using the Plackett-Burman model and Response surface methodology. The traditional one-factor-at-a-time optimization approach is laborious, time-consuming, and requires more experiments which makes the process and analysis more difficult. The Designed PB model was found to be significant for biomass (396 mg/L), lipid (254 mg/L), and EPA (5.6%) production with a P value < 0.05. The major objective of this study is to formulate a medium for EPA production without compromising the growth properties. Further, we had formulated a new media using RSM to achieve the goal and the significant variables selected were NaNO3, NaH2PO4, and IAA and was found to be significant with 16.72% EPA production with a biomass production of 893 mg/L with a P value < 0.05. The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s43393-021-00069-1.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s43393-021-00069-1.
摘要：
微藻被认为是高价值代谢物的丰富来源，具有一系列营养和药物应用。已经开发了在大规模光生物反应器中培养微藻的不同策略，但高成本和低生产率是主要障碍。优化用于培养微藻的培养基组成以诱导生物量产生和高价值代谢物积累已被认为是可持续产品开发的重要因素。在这项研究中,植物生长调节剂和基础微藻培养基对生物量的影响，总脂质，使用Plackett-Burman模型和响应面法研究了EPA的生产。传统的单因素一次优化方法是费力的，耗时,并且需要更多的实验，这使得过程和分析更加困难。发现设计的PB模型对生物量(396mg/L)显著，脂质（254毫克/升），和EPA(5.6%)产量，P值<0.05。这项研究的主要目的是在不损害生长特性的情况下配制用于EPA生产的培养基。Further,我们使用RSM配制了一种新的培养基来实现目标，选择的重要变量是NaNO3，NaH2PO4和IAA，发现EPA产量为16.72％，生物量产量为893mg/L，P值<0.05。配制的培养基可用于大规模培养系统，该系统可提高海洋微藻的生物量产量以及omega3脂肪酸产量。
■在线版本包含补充材料，可在10.1007/s43393-021-00069-1获得。
■在线版本包含补充材料，可在10.1007/s43393-021-00069-1获得。
