PDF(Pubmed)
Abstract：
The bioaccessibility and bioavailability of phenolic compounds (PC) influence directly their role in disease prevention/control. Studies have evaluated this ability through complex plant and food matrices, which may reflect more a synergistic effect of the matrix than the ability of the PCs, hindering their individual exploitation in nutraceutical or pharmaceutical applications. In the present study ten pure PCs representing major classes were evaluated for their bioaccessibility and intestinal absorption in an in vitro simulated gastrointestinal digestion (SGD). This is the first study concerning the bioaccessibility evaluation of pure phloretin, phloroglucinol, naringin, naringenin and daidzein, while no in vitro SGD has been performed before for the other compounds considered here. PCs were analyzed through ultra-high-performance liquid chromatography coupled with diode-array detection and tandem mass spectrometry (UHPLC-DAD-MSn). Most of the compounds remained present along the gastrointestinal tract, and the bioaccessibility was in general higher than 50%, except for quercetin, epigallocatechin gallate, and ellagic acid. All compounds were highly absorbed in the intestine, with phloretin showing the lowest percentage at about 82%. The study findings provide new knowledge on the bioaccessibility and intestinal absorption of different PCs classes.
摘要：
酚类化合物（PC）的生物可及性和生物利用度直接影响其在疾病预防/控制中的作用。研究通过复杂的植物和食物基质评估了这种能力，这可能比PC的能力更能反映基质的协同效应，阻碍他们在营养品或药物应用中的个人开发。在本研究中，评估了代表主要类别的十种纯PC在体外模拟胃肠道消化（SGD）中的生物可利用性和肠道吸收。这是第一个关于纯根皮素生物可及性评价的研究，间苯三酚,柚皮苷,柚皮素和Daidzein,而之前没有对本文考虑的其他化合物进行体外SGD。通过超高效液相色谱结合二极管阵列检测和串联质谱(UHPLC-DAD-MSn)分析PC。大多数化合物仍然存在于胃肠道，生物可及性一般高于50%，除了槲皮素,表没食子儿茶素没食子酸酯，还有鞣花酸.所有化合物在肠道中高度吸收，根皮素的百分比最低，约为82%。研究结果为不同PC类别的生物可及性和肠道吸收提供了新的知识。
