Abstract：
Microglia and oligodendrocyte precursor cells (OPCs) are critical glia subsets in the central nervous system (CNS) and are actively engaged in a body of diseases, such as stroke, Alzheimer\'s disease, multiple sclerosis, etc. Microglia and OPC serve as compelling tools for the study of CNS diseases as well as the repair and damage of myelin sheath in vitro. In this protocol, we summarized a method which is capable of using the same batch of new-born mice to isolate and culture microglia and OPCs. It integrates the characteristics of practicality, convenience, and efficiency, providing a convenient, easy, and reliable technique for research.
摘要：
小胶质细胞和少突胶质细胞前体细胞（OPCs）是中枢神经系统（CNS）中重要的神经胶质细胞亚群，并积极参与一系列疾病，如中风，老年痴呆症,多发性硬化症,等。小胶质细胞和OPC是研究中枢神经系统疾病以及体外髓鞘修复和损伤的重要工具。在这个协议中,我们总结了一种能够使用同一批新生小鼠分离和培养小胶质细胞和OPC的方法。它融合了实用性的特点,便利性，和效率，提供方便，easy,和可靠的研究技术。
