Abstract：
Lignocellulose biorefinery depended on effective pretreatment strategies is of great significance for solving the current global crisis of ecosystem and energy security. This study proposes a novel approach combining seawater hydrothermal pretreatment (SHP) and microwave-assisted deep eutectic solvent (MD) pretreatment to achieve an effective fractionation of Pinus massoniana into high value-added products. The results indicated that complex ions (Mg2+, Ca2+, and Cl-) in natural seawater served as Lewis acids and dramatically promoted the depolymerization of mannose and xylan into oligosaccharides with 40.17 % and 75.43 % yields, respectively. Subsequent MD treatment realized a rapid and effective lignin fractionation (~90 %) while retaining cellulose. As a result, the integrated pretreatment yielded ~85 % of enzymatic glucose, indicating an eightfold increase compared with untreated pine. Because of the increased hydrophobicity induced by the formation of acyl groups during MD treatment, uniform lignin nanospheres were successfully recovered from the DES. It exhibited low dispersibility (PDI = 2.23), small molecular weight (1889 g/mol), and excellent oxidation resistance (RSI = 5.94), demonstrating promising applications in functional materials. The mechanism of lignin depolymerization was comprehensively elucidated via FTIR, 2D-HSQC NMR, and GPC analyses. Overall, this study provides a novel and environmentally friendly strategy for lignocellulose biorefinery and lignin valorization.
摘要：
木质纤维素生物炼制依赖于有效的预处理策略对于解决当前全球生态系统和能源安全危机具有重要意义。本研究提出了一种结合海水水热预处理（SHP）和微波辅助深度共晶溶剂（MD）预处理的新方法，以实现马尾松有效分馏为高附加值产品。结果表明,复合离子(Mg2+,Ca2+,和Cl-）在天然海水中充当路易斯酸，并显着促进甘露糖和木聚糖解聚为寡糖，产率为40.17％和75.43％，分别。随后的MD处理实现了快速有效的木质素分馏(~90%),同时保留了纤维素。因此,综合预处理产生约85%的酶促葡萄糖，表明与未经处理的松树相比增加了八倍。由于在MD处理期间由酰基的形成引起的疏水性增加,从DES中成功地回收了均匀的木质素纳米球。它表现出低分散性（PDI=2.23），小分子量（1889克/摩尔），和优异的抗氧化性（RSI=5.94），在功能材料中展示了有希望的应用。通过FTIR对木质素解聚机理进行了全面的阐明,2D-HSQCNMR,和GPC分析。总的来说,本研究为木质纤维素生物炼制和木质素增值提供了一种新的环保策略。
