PDF(Pubmed)
Abstract：
The zebrafish is a powerful model organism for studying development and regeneration. However, there is a lack of a standardized reference diet for developmental and regeneration experiments. Most studies evaluate the rate of growth, survival, and fecundity. In this study, we compare three diets and their effects on growth and regeneration after a spinal cord injury (SCI). Fish were fed daily for 1 week with daily measurements of overall length and width of spinal injury. Fish fed a live rotifer diet grew 32%, whereas a commercially available diet only led to a 4% increase in body length. Similarly, differences in rate of regeneration were observed with over 80% of rotifer-fed larvae forming a glial bridge after injury compared to <10% of zebrafish fed with the commercial diet. Our data highlight the need for establishing a standardized diet for regeneration studies to improve research reproducibility.
摘要：
斑马鱼是研究发育和再生的强大模型生物。然而,缺乏发育和再生实验的标准化参考饮食。大多数研究评估的增长率，生存,和繁殖力。在这项研究中,我们比较了三种饮食及其对脊髓损伤（SCI）后生长和再生的影响。每天喂食鱼1周，每天测量脊髓损伤的总长度和宽度。饲喂活轮虫饮食的鱼增长了32％，而商业饮食仅导致体长增加4％。同样，与<10％的商业饮食喂养的斑马鱼相比，超过80％的轮虫喂养的幼虫在受伤后形成神经胶质桥，观察到再生率的差异。我们的数据强调了为再生研究建立标准化饮食以提高研究可重复性的必要性。
