Abstract：
Two Gram-stain-negative bacterial strains, R39T and R73T, were isolated from the rhizosphere soil of the selenium hyperaccumulator Cardamine hupingshanesis in China. Strain R39T transformed selenite into elemental and volatile selenium, whereas strain R73T transformed both selenate and selenite into elemental selenium. Phylogenetic and phylogenomic analyses indicated that strain R39T belonged to the genus Achromobacter, while strain R73T belonged to the genus Buttiauxella. Strain R39T (genome size, 6.68 Mb; G+C content, 61.6 mol%) showed the closest relationship to Achromobacter marplatensis LMG 26219T and Achromobacter kerstersii LMG 3441T, with average nucleotide identity (ANI) values of 83.6 and 83.4 %, respectively. Strain R73T (genome size, 5.22 Mb; G+C content, 50.3 mol%) was most closely related to Buttiauxella ferragutiae ATCC 51602T with an ANI value of 86.4 %. Furthermore, strain A111 from the GenBank database was found to cluster with strain R73T within the genus Buttiauxella through phylogenomic analyses. The ANI and digital DNA-DNA hybridization values between strains R73T and A111 were 97.5 and 80.0% respectively, indicating that they belong to the same species. Phenotypic characteristics also differentiated strain R39T and strain R73T from their closely related species. Based on the polyphasic analyses, strain R39T and strain R73T represent novel species of the genera Achromobacter and Buttiauxella, respectively, for which the names Achromobacter seleniivolatilans sp. nov. (type strain R39T=GDMCC 1.3843T=JCM 36009T) and Buttiauxella selenatireducens sp. nov. (type strain R73T=GDMCC 1.3636T=JCM 35850T) are proposed.
摘要：
两种革兰氏染色阴性细菌菌株，R39T和R73T,从中国硒高积累的卡米米·胡平山西的根际土壤中分离出来。菌株R39T将亚硒酸盐转化为元素和挥发性硒，而菌株R73T将硒酸盐和亚硒酸盐转化为元素硒。系统发育和系统基因组分析表明，菌株R39T属于无色杆菌属，而菌株R73T属于Buttiauxella属。菌株R39T(基因组大小，6.68Mb；G+C含量，61.6mol％）显示出最接近的关系，与加拿大白斑无色杆菌LMG26219T和德国白斑无色杆菌LMG3441T，平均核苷酸同一性（ANI）值为83.6％和83.4％，分别。菌株R73T(基因组大小，5.22Mb；G+C含量，50.3mol％）与产铁牛乳ATCC51602T最密切相关，ANI值为86.4％。此外，通过系统基因组分析，发现GenBank数据库中的A111菌株与Buttiauxella属内的R73T菌株聚类。菌株R73T和A111之间的ANI和数字DNA-DNA杂交值分别为97.5和80.0％，表明它们属于同一物种。表型特征也将菌株R39T和菌株R73T从它们密切相关的物种中区分出来。根据多相分析，菌株R39T和菌株R73T代表无色杆菌属和Buttiauxella属的新物种，分别,其名称为Seleniivolotlans无色杆菌sp。11月。（类型菌株R39T=GDMCC1.3843T=JCM36009T）和硒化布托氏菌。11月。(型应变R73T=GDMCC1.3636T=JCM35850T)提出。
