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Abstract：
Background Stress affects mental health significantly and is a ubiquitous feature of contemporary living. Among the possible antibiotics are omadacycline and vancomycin, whose anti-inflammatory properties have also been thoroughly documented in recent research. The goal of the current study was to examine their complex involvement in the brain\'s stress response circuits and how they modulate stress. An established model organism that provides a useful platform for examining stress-induced behaviors and possible therapeutic approaches is the zebrafish. To investigate how dopamine affects the stress response, we used a zebrafish model that was exposed to stress. Methodology For three minutes, zebrafish were continually subjected to chasing stress. They were then given antibiotic combinations of 50 µg/mL each of vancomycin and omadacycline at various ratios of 1:1, 3:1, and 3:1. Behavior alterations, including freezing bouts, top-bottom ratios, and latency periods, were analyzed and contrasted with control groups. ImageJ software was utilized to analyze the video footage of the fish. Results The study showed that the combination of omadacycline and vancomycin greatly reduced the behaviors in zebrafish caused by stress. They chose their concentration (50 µg/mL) according to the lethal concentration 50% result. By shortening the latency time and increasing the intensity of breezing sessions, these chemicals restored almost normal activity. There was statistical significance in the outcomes. The results show that the combination of vancomycin and omadacycline may have an anti-psychotic impact on zebrafish behaviors brought on by stress. Their control of stress reactions is consistent with their known roles in the reward and stress circuits of the brain. These results emphasize the complex interactions between neurotransmitter systems and the control of stress, highlighting the therapeutic potential of dopamine in the treatment of stress-related mental illnesses. Conclusions The combination of vancomycin and omadacycline has been shown to have anti-psychotic effects, which presents potential opportunities for the development of new treatment strategies for mental diseases associated with stress. To fully understand the specific processes underpinning their involvement in stress management and how they relate to mental illnesses in humans, more investigation is necessary.
摘要：
背景压力显着影响心理健康，是当代生活中普遍存在的特征。可能的抗生素包括奥马环素和万古霉素，其抗炎特性在最近的研究中也得到了彻底的证明。当前研究的目的是检查他们在大脑的应激反应回路中的复杂参与以及他们如何调节压力。斑马鱼是一种已建立的模型生物，为检查应激诱导的行为和可能的治疗方法提供了有用的平台。为了研究多巴胺如何影响应激反应，我们使用了暴露在压力下的斑马鱼模型。方法三分钟，斑马鱼不断受到追逐压力。然后以1:1、3:1和3:1的不同比例给予万古霉素和奥马环素各50μg/mL的抗生素组合。行为改变，包括冻结的比赛，上下比率,和延迟周期，并与对照组进行对比分析。利用ImageJ软件分析鱼的视频片段。结果奥马环素与万古霉素合用可显著降低斑马鱼应激行为。他们根据致死浓度50％结果选择其浓度（50µg/mL）。通过缩短延迟时间和增加微风会话的强度，这些化学物质几乎恢复正常活动。结果有统计学意义。结果表明，万古霉素和omadacycline的组合可能对应激引起的斑马鱼行为产生抗精神病作用。他们对应激反应的控制与他们在大脑的奖励和压力回路中的已知作用一致。这些结果强调了神经递质系统和压力控制之间的复杂相互作用，强调多巴胺在治疗压力相关精神疾病中的治疗潜力。结论万古霉素和奥马环素联合用药具有抗精神病作用,这为开发与压力相关的精神疾病的新治疗策略提供了潜在的机会。为了充分了解他们参与压力管理的具体过程，以及它们与人类精神疾病的关系，更多的调查是必要的。
