PDF(Pubmed)
Abstract：
Mucormycosis usually occurs in immunocompromised patients or those with uncontrolled diabetes. Along the third wave of SARS-CoV-2, an associated angioinvasive opportunistic infection with Mucor, a life-threatening fungal infection, was rampant and emerging. With an increase in the usage of steroids in the COVID scenario, the rate of mucormycosis did take a rapid and alarming increase in King Edward Memorial Hospital, Pune, India. Any delay in the diagnosis and management of the disease was life-threatening. The most conventional methods to diagnose mucormycosis are microbiological culture and histopathology of the tissue. The microbiological culture method plays an important role in the diagnosis of mucormycosis. However, the technique is labour-intensive, taking seven to eight days. Histopathology leads to false-negative reports if the tissue is not biopsied from representative sites. On the other hand, molecular methods are rapid, reliable, and applicable to different body samples, such as tissue, paraffin-embedded tissue blocks, plasma, and urine. We aimed to use a reverse transcriptase polymerase chain reaction (RT-PCR) method to detect Mucor in plasma samples. Due to a lack of availability of fresh samples, nucleic acid was extracted from the tissue sections of 69 cases diagnosed as Mucor by histopathology. These samples were subjected to RT-PCR using the MucorGenius kit (Pathonostics, Maastricht, Netherlands). A total of 57 tissue samples were sent for culture, and 49% of our cases were positive by culture and equally by RT-PCR. There was 80% sensitivity and 76% specificity between culture and PCR techniques. However, the use of blood/plasma for RT-PCR for early diagnosis of mucormycosis will be the method of choice.
摘要：
毛霉菌病通常发生在免疫功能低下的患者或患有不受控制的糖尿病的患者中。沿着SARS-CoV-2的第三波，与Mucor相关的血管侵袭性机会性感染，威胁生命的真菌感染,正在猖獗和崛起。随着COVID场景中类固醇使用量的增加，爱德华国王纪念医院的毛霉菌病发病率迅速而惊人地上升，浦那,印度。该疾病的诊断和管理的任何延误都会危及生命。诊断毛霉菌病的最常规方法是微生物培养和组织病理学。微生物培养方法在毛霉菌病的诊断中起着重要作用。然而,这项技术是劳动密集型的，需要7到8天。如果组织未从代表性部位进行活检，则组织病理学会导致假阴性报告。另一方面,分子方法是快速的，可靠,适用于不同的身体样本，如组织，石蜡包埋的组织块,等离子体,还有尿液.我们旨在使用逆转录酶聚合酶链反应（RT-PCR）方法检测血浆样品中的Mucor。由于缺乏新鲜样品，从69例经组织病理学诊断为Mucor的组织切片中提取核酸。这些样品使用MucorGenius试剂盒进行RT-PCR（病理学，马斯特里赫特,荷兰)。共有57份组织样本送去培养,49％的病例通过培养和RT-PCR均为阳性。培养和PCR技术之间有80%的灵敏度和76%的特异性。然而,使用血液/血浆进行RT-PCR早期诊断毛霉菌病将是首选方法.
